Jann K, Jann B, Winter V, Wolf-Ullisch C
J Gen Microbiol. 1979 Jan;110(1):203-10. doi: 10.1099/00221287-110-1-203.
The O8 and O9-specific lipopolysaccharides of Escherichia coli lost their serological activity during liberation of the polysaccharide moieties (alpha-mannans) by mild acid hydrolysis, as tested by passive haemagglutination and haemagglutination inhibition. The serological activities and specificities were restored by substitution of the polysaccharides with 1 to 2 stearoyl groups per polysaccharide chain. The mannans obtained by biosynthesis in vitro were serologically active only when bound to the membrane-associated hydrophobic carrier molecule. Liberation of the polysaccharides from the carrier by treatment with aqueous phenol resulted in loss of the serological activity. The O8- and O9-specific mannans of E. coli are thus serologically active when they are part of an amphiphilic molecule and not as free polysaccharides.
通过被动血凝和血凝抑制试验检测发现，大肠杆菌O8和O9特异性脂多糖在经温和酸水解释放多糖部分（α-甘露聚糖）的过程中失去了血清学活性。通过每条多糖链用1至2个硬脂酰基团取代多糖，血清学活性和特异性得以恢复。体外生物合成得到的甘露聚糖只有在与膜相关的疏水载体分子结合时才具有血清学活性。用水相苯酚处理使多糖从载体上释放出来，导致血清学活性丧失。因此，大肠杆菌的O8和O9特异性甘露聚糖作为两亲分子的一部分时具有血清学活性，而作为游离多糖时则没有。
