与L细胞病毒粒子相关的一种异常DNA聚合酶活性的证明。

Demonstration of an unusual DNA polymerase activity associated with the L cell virion.

作者信息

Hallinan F M, Lee S H, Rozee K R

出版信息

J Gen Virol. 1979 Mar;42(3):521-32. doi: 10.1099/0022-1317-42-3-521.

Abstract

Purified preparations of L cell virions (LCV) were found to possess an associated DNA polymerase activity. This enzyme was active with poly(C).oligo(dG) and poly(Cm).oligo(dG) and was able to transcribe poly(A).oligo(dT). Endogenous DNA synthesis was also demonstrable in disrupted virion preparations but this reaction was enhanced, rather than inhibited, by RNase pre-treatment. The effects of variations in a number of the assay parameters on these activities were examined in an attempt to determine the class of DNA polymerase involved.

摘要

已发现L细胞病毒粒子（LCV）的纯化制剂具有相关的DNA聚合酶活性。这种酶对聚（C）·寡聚（dG）和聚（Cm）·寡聚（dG）有活性，并且能够转录聚（A）·寡聚（dT）。在破碎的病毒粒子制剂中也可证明内源性DNA合成，但核糖核酸酶预处理增强而非抑制了该反应。研究了一些测定参数变化对这些活性的影响，试图确定所涉及DNA聚合酶的类别。