Kalter S S, Heberling R L, Barry J D, Tian P Y
Virus Reference Laboratory, Inc., San Antonio, TX 78229, USA.
Lab Anim Sci. 1995 Oct;45(5):523-5.
Thirty human and nonhuman primate sera tested at the Centers for Disease Control by enzyme-linked immunosorbent assay (ELISA), immunofluorescent antibody assay (IFA), and Western blotting were retested at the Virus Reference Laboratory, Inc. by the dot-immunobinding assay (DIA). The Ebola-Reston strain of virus received from the Centers for Disease Control was prepared into a suitable DIA antigen as described for other antigens. All six Western blotting-positive sera were also positive by DIA, as were the five ELISA-positive sera. Testing by IFA, the original test of choice, indicated an additional four seropositives, all negative by the other test systems. Of 288 randomly selected macaque sera, 19 were also found to be Ebola-Reston virus-positive by DIA.
疾病控制中心采用酶联免疫吸附测定法（ELISA）、免疫荧光抗体测定法（IFA）和蛋白质印迹法对30份人类和非人类灵长类动物血清进行了检测，病毒参考实验室有限公司又采用斑点免疫结合测定法（DIA）对这些血清进行了重新检测。从疾病控制中心收到的埃博拉 - 莱斯顿病毒株按照其他抗原的描述制备成合适的DIA抗原。所有6份蛋白质印迹法检测呈阳性的血清经DIA检测也呈阳性，5份ELISA检测呈阳性的血清同样如此。采用最初的首选检测方法IFA检测时，又发现了4份血清呈阳性，而在其他检测系统中这4份血清均呈阴性。在随机选取的288份猕猴血清中，经DIA检测发现有19份血清埃博拉 - 莱斯顿病毒呈阳性。
