Huynh-ngoc T, Sirois G
J Pharm Sci. 1977 Apr;66(4):591-2. doi: 10.1002/jps.2600660436.
Different methods for the spectrofluorometric determination of quinidine in plasma and urine were studied. The alkaline washings of plasma and urine extracts remove fluorescent metabolites, as shown by TLC analysis of urine extracts, and do not lead to a significant loss of alkaloids. The spectrofluormetric assays without alkaline washings of the benzene extract averaged 18% higher than the assays with alkaline washings. Since unchanged quinidine and hydroquinidine are responsible for antiarrhythmic activity, the method with alkaline washing is more appropriate for the control of quinidinemia than are other methods. The therapeutic plasma concentration range becomes 0.8-2.5 microng/ml with this methods.
研究了血浆和尿液中奎尼丁的荧光分光光度测定的不同方法。血浆和尿液提取物的碱性洗涤可去除荧光代谢物，尿液提取物的薄层色谱分析表明了这一点，并且不会导致生物碱的显著损失。未进行苯提取物碱性洗涤的荧光分光光度测定平均比进行碱性洗涤的测定高18%。由于未改变的奎尼丁和氢奎尼丁具有抗心律失常活性，因此与其他方法相比，碱性洗涤方法更适合用于监测奎尼丁血症。采用该方法时，治疗性血浆浓度范围为0.8 - 2.5微克/毫升。
