Schmalreck A F, Kottmann I, Reiser A, Ruffer U, Scharr E, Vanca E
Pfizer/Mack Laboratories-Microbiology R&D, Illertissen, Germany.
Mycoses. 1995 Sep-Oct;38(9-10):359-68. doi: 10.1111/j.1439-0507.1995.tb00065.x.
Four commercially available in vitro test systems (Candifast, E-test, Mycototal and spiral-gradient end point method), agar diffusion with 25-micrograms fluconazole paper test discs and 15-micrograms test tablets and agar dilution were compared with the microbroth dilution method for fluconazole susceptibility testing of 145 clinical isolates. In addition, the culture media provided or recommended by the manufacturers of the test systems were compared with high-resolution (HR) antifungal test medium. With all currently available culture media, growth problems (inhibition or delayed growth of the clinical isolates) occurred with solid or semisolid media. With minor improvements, HR medium demonstrated the most reproducible and comparable results (supplementation with asparagine and deletion of sodium hydrogen carbonate). The best correlation with microdilution was obtained by the agar dilution method (> 95% concordance) followed by the spiral-gradient end point method (85%), Candifast (83%), Mycototal (81%) and the E-test (78%). Regression analysis demonstrated good correlation between agar diffusion and micro-/agar dilution (r > 0.9).
将四种市售体外测试系统（Candifast、E-test、Mycototal和螺旋梯度终点法）、含25微克氟康唑纸片的琼脂扩散法、含15微克测试片的琼脂扩散法以及琼脂稀释法，与微量肉汤稀释法进行比较，以检测145株临床分离株对氟康唑的敏感性。此外，还将测试系统制造商提供或推荐的培养基与高分辨率（HR）抗真菌测试培养基进行了比较。使用所有现有的培养基时，固体或半固体培养基均出现生长问题（临床分离株生长受到抑制或延迟）。经过微小改进后，HR培养基显示出最可重复和可比的结果（添加天冬酰胺并去除碳酸氢钠）。与微量稀释法相关性最好的是琼脂稀释法（一致性>95%），其次是螺旋梯度终点法（85%）、Candifast（83%）、Mycototal（81%）和E-test（78%）。回归分析表明琼脂扩散法与微量/琼脂稀释法之间具有良好的相关性（r>0.9）。
