Schmalreck A F
Pfizer/Mack, Mikrobiologie Forschung und Entwicklung, Illertissen, BR Deutschland.
Mycoses. 1996;39 Suppl 2:1-11. doi: 10.1111/j.1439-0507.1996.tb00520.x.
In a collaborative study for in vitro testing of fluconazole against clinical yeast isolates participated 21 laboratories of the working group "Clinical Mycology" of the German Speaking Mycological Society. In these centers, according to a standard test protocol 1181 clinical isolates from 1033 patients were tested to their susceptibility against fluconazole by microdilution, agar diffusion and partly by agar dilution. Approximately 600 strains (59.1%) of the collective of 1106 isolates sent to a reference center underwent retesting in one laboratory (center 13). These strains demonstrated almost the same species distribution as the total collective. For 80% of all isolates a MIC of < or = 4 micrograms/ml and for 90% of the Candida albicans strains a MIC of < or = 2 micrograms/ml has been determined. Only approx, 9% of all isolates (4% with Candida albicans) showed a MIC of < or = 25 micrograms/ml. By parallel testing of 10 control strains issued by the reference center to the laboratories, the inter- and intra-laboratory comparability of the susceptibility testing of fluconazole was checked. The results demonstrated that under appropriate technical prerequisites and standardised test conditions, the methods used routinely in bacteriology microdilution, agar dilution and agar diffusion may also be applied in a reproducible way in the routine mycological laboratory for the susceptibility testing of yeasts.
在一项关于氟康唑针对临床酵母分离株的体外测试的合作研究中，德语真菌学协会“临床真菌学”工作组的21个实验室参与其中。在这些中心，按照标准测试方案，对来自1033名患者的1181株临床分离株进行了氟康唑敏感性测试，测试方法包括微量稀释法、琼脂扩散法，部分还采用了琼脂稀释法。送往一个参考中心的1106株分离株中，约600株（59.1%）在一个实验室（13号中心）进行了复测。这些菌株的物种分布与整个集合几乎相同。已确定所有分离株中80%的最低抑菌浓度（MIC）≤4微克/毫升，白色念珠菌菌株中90%的MIC≤2微克/毫升。所有分离株中只有约9%（白色念珠菌为4%）的MIC≤25微克/毫升。通过对参考中心发放给各实验室的10株对照菌株进行平行测试，检验了氟康唑敏感性测试在实验室间和实验室内部的可比性。结果表明，在适当的技术条件和标准化测试条件下，细菌学中常规使用的微量稀释法、琼脂稀释法和琼脂扩散法也可在常规真菌学实验室以可重复的方式用于酵母的敏感性测试。
