Schmalreck A F, Kottmann I, Reiser A, Ruffer U, Scharr E, Vanca E
Microbiology R&D, Pfizer/Mack Laboratories, Illertissen, BR Deutschland.
Mycoses. 1995;38 Suppl 1:55-63. doi: 10.1111/j.1439-0507.1995.tb00486.x.
Four commercially available in vitro test systems (Candifast, E-test, Mycototal, Spiral-Gradient Endpoint Method), agardiffusion with 25 micrograms fluconazole paper test discs and 15 micrograms test tablets, and agardilution were compared to the microbroth dilution method by fluconazole susceptibility testing of 145 clinical isolates. In addition, the culture media provided or recommended by the manufacturers of the test systems were compared to the high resolution (HR) antifungal test medium. With all currently available culture media growth problems (inhibition or delayed growth of the clinical isolates) occurred with solid or semi-solid media. With minor improvements, HR medium demonstrated the most reproducible and comparable results (supplementation with asparagine and deletion of sodium hydrogen carbonate). Best correlation to microdilution was obtained by the agardilution method > (95% concordance) followed by the spiral gradient endpoint method (85%), Candifast (83%), Mycototal (81%) and the E-test (78%). Regression analysis demonstrated good correlation between agardiffusion and micro-/agardilution(r > 0.9).
通过对145株临床分离株进行氟康唑敏感性测试，将四种市售体外测试系统（Candifast、E-test、Mycototal、螺旋梯度终点法）、含25微克氟康唑纸片的琼脂扩散试验以及含15微克测试片的琼脂扩散试验和琼脂稀释法与微量肉汤稀释法进行了比较。此外，还将测试系统制造商提供或推荐的培养基与高分辨率（HR）抗真菌测试培养基进行了比较。使用所有目前可用的培养基时，固体或半固体培养基均出现了生长问题（临床分离株生长受到抑制或延迟）。经过微小改进后，HR培养基显示出最可重复和可比的结果（添加天冬酰胺并去除碳酸氢钠）。与微量稀释法相关性最好的是琼脂稀释法（一致性>95%），其次是螺旋梯度终点法（85%）、Candifast（83%）、Mycototal（81%）和E-test（78%）。回归分析表明琼脂扩散法与微量/琼脂稀释法之间具有良好的相关性（r>0.9）。
