Vann J M, Goldman A J, Szurek P F, Brooks B R
Neurology Service, William S. Middleton Memorial Veterans Hospital, Madison, WI 53705-2286, USA.
Neurochem Res. 1995 Sep;20(9):1013-20. doi: 10.1007/BF00995554.
Deoxyglucose uptake by FVB/N mouse astrocytes was studied before and after infection by ts1 retrovirus which causes a neurodegenerative disease in mice similar to HIV-1 encephalopathy in man. The Michaelis-Menten kinetic parameters, Km and Vmax, of 2-deoxy-D-glucose uptake by brain and cerebellar astrocytes were measured following culture at 34 degrees C where ts1 retrovirus replicates optimally, and at 37 degrees C. Compared to astrocytes cultured at 37 degrees C, astrocytes cultured at 34 degrees C had increased Km and decreased deoxyglucose uptake despite increased or unchanged Vmax. Following ts1 retrovirus infection, brain astrocyte deoxyglucose uptake doubled [132%] associated with decreased Km but unchanged Vmax, whereas cerebellar astrocyte deoxyglucose uptake doubled [102%] associated with increased Vmax but unchanged Km. These observations of altered deoxyglucose uptake kinetic parameters following retrovirus infection indicate different neurochemical mechanisms for the regional variation in deoxyglucose uptake observed following retrovirus infection of the CNS in vivo.
研究了ts1逆转录病毒感染FVB/N小鼠星形胶质细胞前后的脱氧葡萄糖摄取情况。ts1逆转录病毒可在小鼠中引发一种类似于人类HIV-1脑病的神经退行性疾病。在34℃（ts1逆转录病毒最佳复制温度）和37℃培养后，测量了脑和小脑星形胶质细胞摄取2-脱氧-D-葡萄糖的米氏动力学参数Km和Vmax。与在37℃培养的星形胶质细胞相比，在34℃培养的星形胶质细胞Km增加，脱氧葡萄糖摄取减少，尽管Vmax增加或不变。ts1逆转录病毒感染后，脑星形胶质细胞的脱氧葡萄糖摄取增加了一倍[132%]，同时Km降低但Vmax不变，而小脑星形胶质细胞的脱氧葡萄糖摄取增加了一倍[102%]，同时Vmax增加但Km不变。这些关于逆转录病毒感染后脱氧葡萄糖摄取动力学参数改变的观察结果表明，在体内逆转录病毒感染中枢神经系统后观察到的脱氧葡萄糖摄取区域差异存在不同的神经化学机制。
