Lloyd D C, Dolphin G W, Purrott R J, Tipper P A
Mutat Res. 1977 Mar;42(3):401-12. doi: 10.1016/s0027-5107(77)80045-6.
The extent to which X-ray induced mitotic delay at 150 and 400 rad influences chromosome aberration yields was examined in human peripheral blood lymphocytes. The dicentric was used as a marker and aberrations yields were obtained for mixed cultures prepared from equal numbers of normal and irradiated cells. The cultures were terminated following incubation times of 36-120 h. Greater mitotic delay of the order of a few hours was observed at the higher dose. However most reduction in the number of lymphocytes arriving at metaphase by 48 h may be ascribed to interphase death or failure to transform. Analysis of the dicentric distributions which were expected to follow Poisson statistics indicated that cells containing dicentrics were delayed relative to irradiated but aberration-free cells. Cells with one dicentric moved more easily through the first cell cycle than cells containing two dicentrics. Following accidental partial body irradiation, selection in culture favouring the unirradiated lymphocytes does not distort the aberration yield sufficiently to warrant incubation times in excess of the standard 48-52 h.
在人外周血淋巴细胞中，研究了150拉德和400拉德的X射线诱导的有丝分裂延迟对染色体畸变率的影响程度。以双着丝粒作为标记，从等量的正常细胞和受照射细胞制备混合培养物，得出畸变率。培养物在孵育36 - 120小时后终止。在较高剂量下观察到了几小时的更大有丝分裂延迟。然而，到48小时时到达中期的淋巴细胞数量的大多数减少可能归因于间期死亡或未能转化。对预期遵循泊松统计的双着丝粒分布的分析表明，含有双着丝粒的细胞相对于受照射但无畸变的细胞延迟了。含有一个双着丝粒的细胞比含有两个双着丝粒的细胞更容易通过第一个细胞周期。在意外局部身体照射后，培养中有利于未受照射淋巴细胞的选择不会使畸变率产生足够大的扭曲，从而需要超过标准的48 - 52小时的孵育时间。
