Leong J A, Garapin A C, Jackson N, Fanshier L, Levinson W, Bishop J M
J Virol. 1972 Jun;9(6):891-902. doi: 10.1128/JVI.9.6.891-902.1972.
Cells producing Rous sarcoma virus contain virus-specific ribonucleic acid (RNA) which can be identified by hybridization to single-stranded deoxyribonucleic acid (DNA) synthesized with RNA-directed DNA polymerase. Hybridization was detected by either fractionation on hydroxyapatite or hydrolysis with single strand-specific nucleases. Similar results were obtained with both procedures. The hybrids formed between enzymatically synthesized DNA and viral RNA have a high order of thermal stability, with only minor evidence of mismatched nucleotide sequences. Virus-specific RNA is present in both nuclei and cytoplasm of infected cells. This RNA is remarkably heterogeneous in size, including molecules which are probably restricted to the nucleus and which sediment in their native state more rapidly than the viral genome. The nature of the RNA found in cytoplasmic fractions varies from preparation to preparation, but heterogeneous RNA (ca. 4-50S), smaller than the viral genome, is always present in substantial amounts.
产生劳氏肉瘤病毒的细胞含有病毒特异性核糖核酸（RNA），这种RNA可通过与用RNA指导的DNA聚合酶合成的单链脱氧核糖核酸（DNA）杂交来鉴定。通过在羟基磷灰石上分级分离或用单链特异性核酸酶水解来检测杂交。两种方法都得到了相似的结果。酶促合成的DNA与病毒RNA之间形成的杂交体具有很高的热稳定性，只有少量错配核苷酸序列的证据。病毒特异性RNA存在于受感染细胞的细胞核和细胞质中。这种RNA在大小上非常不均一，包括可能局限于细胞核的分子，这些分子在天然状态下的沉降速度比病毒基因组更快。在细胞质组分中发现的RNA的性质因制备方法而异，但总是大量存在比病毒基因组小的不均一RNA（约4-50S）。
