Pillai S D, Widmer K W, Dowd S E, Ricke S C
Environmental Science Program, Texas A&M University Research Center, El Paso, USA.
Appl Environ Microbiol. 1996 Jan;62(1):296-9. doi: 10.1128/aem.62.1.296-299.1996.
Glass impingers (AGI-30) were used at a commercial sludge application site to determine the levels of airborne bacteria and pathogen indicators. Even though heterotrophic bacteria averaged 10(5) CFU/m3, none of the sites showed the presence of Salmonella spp. or indicators such as fecal coliforms or coliphages. Indicators such as H2S producers and pathogenic clostridia were present in locations having significant physical agitation of the sludge material. PCR-based ribotyping using the 16S-23S interspacer region is a promising method to identify the genetic relatedness and origins of airborne clostridia.
在一个商业污泥应用场地使用玻璃撞击式采样器(AGI - 30)来测定空气中细菌和病原体指标的水平。尽管异养细菌平均含量为10⁵CFU/m³,但没有一个场地检测到沙门氏菌属或诸如粪大肠菌群或大肠杆菌噬菌体等指标。在污泥材料有显著物理搅动的位置存在诸如硫化氢产生菌和致病性梭菌等指标。使用16S - 23S间隔区的基于聚合酶链反应的核糖体分型是一种用于鉴定空气中梭菌的遗传相关性和来源的有前景的方法。