Sprenger G A
Institut für Biotechnologie 1, Forschungszentrum Jülich GmbH.
Arch Microbiol. 1995 Nov;164(5):324-30. doi: 10.1007/BF02529978.
The pentose-phosphate pathway of Escherichia coli K-12, in addition to its role as a route for the breakdown of sugars such as glucose or pentoses, provides the cell with intermediates for the anabolism of amino acids, vitamins, nucleotides, and cell wall constituents. Through its oxidative branch, it is a major source of NADPH. The expression of the gene for NADP-dependent 6-phosphogluconate dehydrogenase (gnd) is regulated by the growth rate in E. coli. The recently identified gene for ribulose-5-phosphate 3-epimerase (rpe) is part of a large operon that comprises among other genes for the biosynthesis of aromatic amino acids. In recent years, genes for all enzymes of the pathway have been cloned and sequenced. Isoenzymes have been found for transketolase (genes tktA and tktB), ribose-5-phosphate isomerase (rpiA and rpiB) and transaldolase (talA and talB).
大肠杆菌K-12的磷酸戊糖途径，除了作为葡萄糖或戊糖等糖类分解途径的作用外，还为细胞提供氨基酸、维生素、核苷酸和细胞壁成分合成代谢的中间体。通过其氧化分支，它是NADPH的主要来源。大肠杆菌中依赖NADP的6-磷酸葡萄糖酸脱氢酶（gnd）基因的表达受生长速率调控。最近鉴定出的核糖-5-磷酸3-表异构酶（rpe）基因是一个大操纵子的一部分，该操纵子还包括其他芳香族氨基酸生物合成的基因。近年来，该途径所有酶的基因已被克隆和测序。已发现转酮醇酶（基因tktA和tktB）、核糖-5-磷酸异构酶（rpiA和rpiB）和转醛醇酶（talA和talB）的同工酶。
