Hoogewerf A, Black D, Proudfoot A E, Wells T N, Power C A
Glaxo Institute for Molecular Biology, Geneva, Switzerland.
Biochem Biophys Res Commun. 1996 Jan 5;218(1):337-43. doi: 10.1006/bbrc.1996.0059.
We have cloned the murine homologue of human CC Chemokine Receptor-4 (CC CKR-4). In equilibrium competition binding assays performed in undifferentiated HL-60 cells transfected with human and murine CC CKR-4 cDNA, the IC50 values for the binding of [125I]macrophage inflammatory protein-1 alpha to human and murine CC CKR-4 were 14.5 +/- 9.0 nM and 10.1 +/- 3.0 nM, respectively, and the IC50 values for the binding of [125I]RANTES to human and murine CC CKR-4 were 9.3 +/- 3.0 nM and 5.7 +/- 2.6 nM, respectively. The cDNA clone for murine CC CKR-4 is 1531 bp, and the largest open reading frame encodes a protein of 360 amino acids that is 85% identical to human CC CKR-4. Murine CC CKR-4 was detected in the thymus and T-cell lines by Northern blot analysis. This first report of direct binding of chemokines to CC CKR-4 demonstrates that the highly homologous human and murine receptors have similar binding characteristics and tissue distribution.
我们克隆了人类CC趋化因子受体4（CC CKR-4）的小鼠同源物。在用人类和小鼠CC CKR-4 cDNA转染的未分化HL-60细胞中进行的平衡竞争结合试验中，[125I]巨噬细胞炎性蛋白-1α与人及小鼠CC CKR-4结合的IC50值分别为14.5±9.0 nM和10.1±3.0 nM，[125I]调节激活正常T细胞表达和分泌因子（RANTES）与人及小鼠CC CKR-4结合的IC50值分别为9.3±3.0 nM和5.7±2.6 nM。小鼠CC CKR-4的cDNA克隆为1531 bp，最大的开放阅读框编码一个360个氨基酸的蛋白质，该蛋白质与人类CC CKR-4有85%的同源性。通过Northern印迹分析在胸腺和T细胞系中检测到小鼠CC CKR-4。趋化因子与CC CKR-4直接结合的这一首次报道表明，高度同源的人类和小鼠受体具有相似的结合特性和组织分布。
