Tosh D, Borthwick E B, Sharp S, Burchell A, Burchell B, Coughtrie M W
Department of Biochemical Medicine, University of Dundee, Ninewells Hospital and Medical School, U.K.
Biochem Pharmacol. 1996 Feb 9;51(3):369-74. doi: 10.1016/0006-2952(95)02163-9.
Sulphotransferase (ST) is a family of enzymes responsible for metabolism and detoxication of endobiotics and xenobiotics. We investigated the hepatic acinar distribution of three sulphotransferases: phenol sulphotransferase (PST), oestrogen sulphotransferase (EST), and hydroxysteroid sulphotransferase (HST) in male and female rat livers by measurement of enzyme activities in isolated periportal and perivenous hepatocytes. The distribution was confirmed by immunohistochemistry. EST activity was located predominantly in the perivenous hepatocytes in male rats but not in female rats, where residual activity is catalysed by another ST. HST activity was not significantly different in periportal and perivenous hepatocytes in either male or female rats. For PST, a more widespread distribution was observed, with slight predominance in the periportal regions. The results indicate heterogeneous distribution of ST isoenzymes in the periportal and perivenous hepatocytes isolated from male and female rat livers.
磺基转移酶（ST）是一类负责内源性物质和外源性物质代谢及解毒的酶。我们通过测量分离的门静脉周围和肝静脉周围肝细胞中的酶活性，研究了三种磺基转移酶：苯酚磺基转移酶（PST）、雌激素磺基转移酶（EST）和羟基类固醇磺基转移酶（HST）在雄性和雌性大鼠肝脏中的肝腺泡分布。通过免疫组织化学证实了这种分布。EST活性主要位于雄性大鼠的肝静脉周围肝细胞中，而在雌性大鼠中则不然，雌性大鼠中的残余活性由另一种ST催化。在雄性或雌性大鼠的门静脉周围和肝静脉周围肝细胞中，HST活性没有显著差异。对于PST，观察到其分布更为广泛，在门静脉周围区域略有优势。结果表明，ST同工酶在从雄性和雌性大鼠肝脏分离的门静脉周围和肝静脉周围肝细胞中分布不均一。
