Iaroslavtseva N G, Ivanov V S, Grebennikova Zh O, Kornilaeva G V, Pashkova T A, Chikin L D, Ostrovskiĭ A G, Andreev S M, Khaitov R M, Karamov E V
Bioorg Khim. 1995 Oct;21(10):752-60.
Reactivity of 26 synthetic peptides that comprise 12 to 26 amino acid residues corresponding to segments of the gag p19, env gp46, and pol proteins of human T-lymphotropic virus type I toward 31 positive sera was studied using enzyme-linked immunosorbent assay. Specific reactivity with high titers of antibodies (presented in reciprocal dilution values) was detected for the synthetic peptides corresponding to fragments 110-130 and 100-130 (titers up to 4050) of p19, 174-197 (up to 800), 186-201 (up to 4050), 191-215 (up to 1350), 242-257 (up to 800), and 272-292 (up to 450) of gp46. Immunoreactivity of seven peptides, fragments of pol-proteins, was weak. New linear epitopes in the regions 145-158, 272-277, and 292-300 of gp46 were detected. In addition, location of the known linear epitopes in p19 and gp46 was refined on the basis of comparative study of overlapping peptides from these proteins.
利用酶联免疫吸附测定法，研究了26种合成肽对31份阳性血清的反应性，这些合成肽由12至26个氨基酸残基组成，对应于I型人类嗜T淋巴细胞病毒的gag p19、env gp46和pol蛋白的片段。检测到与高滴度抗体（以倒数稀释值表示）具有特异性反应的合成肽，这些肽对应于p19的110 - 130和100 - 130片段（滴度高达4050）、gp46的174 - 197（高达800）、186 - 201（高达4050）、191 - 215（高达1350）、242 - 257（高达800）和272 - 292（高达450）片段。7种作为pol蛋白片段的肽的免疫反应性较弱。在gp46的145 - 158、272 - 277和292 - 300区域检测到新的线性表位。此外，基于对这些蛋白重叠肽的比较研究，对p19和gp46中已知线性表位的位置进行了优化。
