Ercoli L, Sarmati L, el-Sawaf G, Cochi S, Lanti T, Iudicone P, Guglielmetti M, Giannini G, Galluzzo C, Tomino C
Department of Public Health and Cellular Biology, University Tor Vergata, Rome, Italy.
AIDS Res Hum Retroviruses. 1995 Oct;11(10):1203-7. doi: 10.1089/aid.1995.11.1203.
Quantitative culture of human immunodeficiency virus (HIV) was performed on 202 plasma samples obtained from asymptomatic and early symptomatic HIV-1 infected patients (mean CD4+ count: 186/mm3) before antiretroviral therapy was started. HIV could be isolated from 84% of the plasma samples (titers ranging from 10(0) to 10(2.75) TCID50/ml). Immune complex dissociated p24 antigen (ICD-p24) was detected in 66% of the samples. Only 23 samples (11%) were negative for both ICD-p24 as well as HIV culture. Discordant results were obtained in 55 samples, and 45 samples negative for ICD-p24 were positive for HIV culture. A significant proportion (42%) of patients that were negative for ICD-p24 belonged to a very advanced group with very low CD4+ cell count. However, almost 90% of these ICD-p24 negative samples were positive for HIV plasma viremia, stressing the value of this virological marker in patients with low CD4+ cell count and without any detectable ICD-p24 antigenemia. HIV-1 RNA was detected in all ICD-p24 negative plasma samples tested by the branched DNA (bDNA) assay. A very good correlation was found between high RNA copy number and HIV plasma isolation in samples obtained from patients with low CD4+ cell count, suggesting that HIV-1 RNA quantitation may also reflect viral infectivity of plasma.
在开始抗逆转录病毒治疗之前，对202份来自无症状和早期有症状的HIV-1感染患者（平均CD4 +细胞计数：186/mm3）的血浆样本进行了人类免疫缺陷病毒（HIV）定量培养。84%的血浆样本中可分离出HIV（滴度范围为10(0)至10(2.75) TCID50/ml）。66%的样本中检测到免疫复合物解离的p24抗原（ICD-p24）。仅23份样本（11%）ICD-p24和HIV培养均为阴性。55份样本结果不一致，45份ICD-p24阴性的样本HIV培养为阳性。ICD-p24阴性的患者中有很大比例（42%）属于CD4 +细胞计数极低的非常晚期组。然而，这些ICD-p24阴性样本中近90%的HIV血浆病毒血症呈阳性，强调了这种病毒学标志物在CD4 +细胞计数低且无任何可检测到的ICD-p24抗原血症患者中的价值。通过分支DNA（bDNA）检测法在所有检测的ICD-p24阴性血浆样本中均检测到HIV-1 RNA。在CD4 +细胞计数低的患者样本中，高RNA拷贝数与HIV血浆分离之间发现了非常好的相关性，表明HIV-1 RNA定量也可能反映血浆的病毒感染性。
