Burr D B, Hooser M
Department of Anatomy and Orthopedic Surgery, Indiana University School of Medicine, Indianapolis.
Bone. 1995 Oct;17(4):431-3. doi: 10.1016/s8756-3282(95)00241-3.
En bloc staining with basic fuchsin has become the method of choice to demonstrate bone microdamage produced in vivo. Several alterations have recently been made to the protocol. This technical note presents the new protocols, which include staining through a graded series of alcohols under vacuum, and eliminating the original evaporation stage in the staining protocol. Reasons for variations in staining quality can be related to inadequate dehydration, failure to stain under vacuum, the source of the basic fuchsin, or the solubility of basic fuchsin in water. The most common reasons for over- or understanding are the time left in stain, and the density of the bone. Limitations to the technique include the fact that probably not all cracks are stained by the technique, and the technique is not useful for studies that involve bone with highly variable densities in a single section.
用碱性品红进行整体染色已成为显示体内产生的骨微损伤的首选方法。最近对该方案进行了一些改动。本技术说明介绍了新的方案，包括在真空下通过一系列分级酒精进行染色，并在染色方案中取消原来的蒸发阶段。染色质量变化的原因可能与脱水不足、真空下染色失败、碱性品红的来源或碱性品红在水中的溶解度有关。染色过度或不足的最常见原因是在染色剂中的停留时间以及骨的密度。该技术的局限性包括可能并非所有裂缝都能被该技术染色，并且该技术对于涉及单个切片中密度高度可变的骨的研究无用。
