Sidqui M, Collin P, Vitte C, Forest N
Laboratoire de Biologie-Odontologie, Institut Biomédical des Cordeliers, Paris, France.
Biomaterials. 1995 Nov;16(17):1327-32. doi: 10.1016/0142-9612(95)91048-4.
The adherence of osteoblast-like cells and the resorption activity of isolated osteoclasts on calcium sulphate hemihydrate (CSH) was investigated. After a 24 h incubation period, alkaline phosphatase staining showed that rat osteoblast-like cells ROS 17/2.8 attached on CSH. Neutral red (NR) and tartrate-resistant acid phosphatase (TRAP) staining revealed that osteoclasts attached on CSH. Furthermore, osteoclasts formed lacunae as revealed by scanning electron microscopy. Although the lacunae formed by osteoclasts on CSH were diverse in shape and form, the most common type had approximately a circular outline, with a well-defined margin similar to those formed on dentine. Less commonly, excavations appeared as a discontinuous area of resorbed CSH with the presence of a circular zone around the non-resorbed area. Finally, by using 10(-9) M calcitonin, evidence was obtained that NR-positive cells were osteoclasts (58.3% and 57.66% decrease of NR-positive mouse and rat cells detected on CSH after 24 h incubation). However, no inhibition was obtained with 10(-11) M calcitonin. The overall number of NR-positive osteoclasts adherent on 256 mm2 CSH was 43 +/- 14 and 42 +/- 3 for mice and rat, respectively. The overall number of TRAP-positive mouse osteoclasts was 67 +/- 12. Acetazolamide (10(-5) M), a carbonic anhydrase inhibitor, inhibited the number of adherent NR osteoclasts on CSH by 50.42% and 41.6% for mouse and rat, respectively. These results indicate that osteoblasts attach on CSH and osteoclasts resorb CSH in vitro.
研究了成骨样细胞在半水硫酸钙（CSH）上的黏附以及分离的破骨细胞对其的吸收活性。孵育24小时后，碱性磷酸酶染色显示大鼠成骨样细胞ROS 17/2.8附着在CSH上。中性红（NR）和抗酒石酸酸性磷酸酶（TRAP）染色显示破骨细胞附着在CSH上。此外，扫描电子显微镜显示破骨细胞形成了陷窝。虽然破骨细胞在CSH上形成的陷窝形状各异，但最常见的类型大致呈圆形轮廓，边缘清晰，类似于在牙本质上形成的陷窝。较少见的情况是，吸收区域表现为CSH吸收的不连续区域，未吸收区域周围有一个圆形区域。最后，使用10(-9)M降钙素，有证据表明NR阳性细胞为破骨细胞（孵育24小时后，在CSH上检测到的NR阳性小鼠和大鼠细胞分别减少了58.3%和57.66%）。然而，10(-11)M降钙素未产生抑制作用。附着在256平方毫米CSH上的NR阳性破骨细胞总数，小鼠为43±14个，大鼠为42±3个。TRAP阳性小鼠破骨细胞总数为67±12个。碳酸酐酶抑制剂乙酰唑胺（10(-5)M）分别使附着在CSH上的NR破骨细胞数量减少了50.42%（小鼠）和41.6%（大鼠）。这些结果表明，成骨细胞可附着在CSH上，破骨细胞可在体外吸收CSH。
