实验性糖尿病大鼠肾脏中晚期糖基化终产物的定位

Localization of advanced glycation endproducts in the kidney of experimental diabetic rats.

作者信息

Shikata K, Makino H, Sugimoto H, Kushiro M, Ota K, Akiyama K, Araki N, Horiuchi S, Ota Z

机构信息

Third Department of Internal Medicine, Okayama University Medical School, Japan.

出版信息

J Diabetes Complications. 1995 Oct-Dec;9(4):269-71. doi: 10.1016/1056-8727(95)80019-b.

DOI:10.1016/1056-8727(95)80019-b

PMID:8573744

Abstract

Advanced glycation endproducts (AGE) have been proposed as a major mediator in the development of various diabetic complications. In order to evaluate the involvement of AGE in the development of diabetic nephropathy, we examined the localization of AGE in the kidney of the streptozotocin-induced diabetic rats immunohistochemically using a monoclonal antibody directed to AGE. In the diabetic rats, glomerular hypertrophy, thickening of the glomerular basement membrane, and expansion of mesangial matrix were observed. AGE was detected in expanded mesangial area and glomerular basement membrane in the kidneys of diabetic rats. The present results suggest that AGE may participate in the development of diabetic nephropathy.

摘要

晚期糖基化终产物（AGE）被认为是各种糖尿病并发症发生发展的主要介质。为了评估AGE在糖尿病肾病发生中的作用，我们使用针对AGE的单克隆抗体，通过免疫组织化学方法检测了链脲佐菌素诱导的糖尿病大鼠肾脏中AGE的定位。在糖尿病大鼠中，观察到肾小球肥大、肾小球基底膜增厚和系膜基质扩张。在糖尿病大鼠肾脏的系膜区扩大和肾小球基底膜中检测到了AGE。目前的结果表明，AGE可能参与了糖尿病肾病的发生发展。