Niwa T, Miyazaki T, Katsuzaki T, Tatemichi N, Takei Y
Nagoya University Branch Hospital, Japan.
Nephron. 1996;74(3):580-5. doi: 10.1159/000189456.
To investigate a role of the Maillard reaction in the pathogenesis of diabetic nephropathy, we measured serum levels of 3-deoxyglucosone (3-DG), a potent protein cross-linking intermediate of the Maillard reaction, and tissue contents of advanced glycation end products (AGEs) in streptozotocin (STZ)-induced diabetic rats. We quantified serum 3-DG using gas chromatography/mass spectrometry, and measured AGE contents in tissues using a competitive enzyme-linked immunosorbent assay with a monoclonal anti-AGE antibody. The STZ-induced diabetic rats showed nephropathy with proteinuria, hypoproteinemia, hyperlipidemia and reduced creatinine clearance. Serum levels of 3-DG in the STZ-induced diabetic rats (mean +/- 3.46 +/- 0.23 mumol/l) were significantly (p < 0.01) higher than those in control rats (1.23 +/- 0.13 mumol/l). AGE contents in the kidney and the lens obtained from the STZ-induced diabetic rats (398 +/- 45 and 816 +/- 200 arbitrary units, respectively) were also significantly (p < 0.01) higher than those in the control rats (122 +/- 10 and 299 +/- 50 arbitrary units, respectively). The results indicate that increased levels of serum 3-DG and renal tissue. AGEs may be related to the occurrence of diabetic nephropathy.
为了研究美拉德反应在糖尿病肾病发病机制中的作用，我们测定了链脲佐菌素（STZ）诱导的糖尿病大鼠血清中3-脱氧葡萄糖酮（3-DG，美拉德反应中一种有效的蛋白质交联中间体）的水平以及晚期糖基化终产物（AGEs）的组织含量。我们使用气相色谱/质谱法定量血清中的3-DG，并使用单克隆抗AGE抗体通过竞争性酶联免疫吸附测定法测量组织中的AGE含量。STZ诱导的糖尿病大鼠出现了伴有蛋白尿、低蛋白血症、高脂血症和肌酐清除率降低的肾病。STZ诱导的糖尿病大鼠血清中3-DG的水平（平均±3.46±0.23μmol/l）显著高于对照组大鼠（1.23±0.13μmol/l）（p<0.01）。从STZ诱导的糖尿病大鼠获取的肾脏和晶状体中的AGE含量（分别为398±45和816±200任意单位）也显著高于对照组大鼠（分别为122±10和299±50任意单位）（p<0.01）。结果表明血清3-DG水平和肾脏组织中AGEs的升高可能与糖尿病肾病的发生有关。
