Qadri F, Mohi G, Hossain J, Azim T, Khan A M, Salam M A, Sack R B, Albert M J, Svennerholm A M
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka, fqadri%
Clin Diagn Lab Immunol. 1995 Nov;2(6):685-8. doi: 10.1128/cdli.2.6.685-688.1995.
Vibrio cholerae serogroup O139, now considered to be the second organism capable of causing epidemic severe dehydrating cholera, contains a capsular polysaccharide which makes it difficult for it to be used in the conventional vibriocidal antibody assay optimized for V. cholerae O1. After modification of the procedure, which involved the use of specific bacterial strains, a lower bacterial inoculum, and increased amounts of complement, the vibriocidal antibody responses to V. cholerae O139 were measured in acute- and convalescent-phase sera from 33 V. cholerae O139-infected and 18 V. cholerae O1-infected patients and in single serum samples from 20 healthy control subjects. The responses in these individuals to V. cholerae O1 strains were also determined. Significant elevations in the homologous antibody response were found only in the convalescent-phase sera from both groups of patients with cholera. These findings may explain the basis for the lack of heterologous protection between the two serogroups of V. cholerae. Healthy controls had higher background levels of vibriocidal antibody to V. cholerae O1 than to V. cholerae O139.
霍乱弧菌O139血清群，现在被认为是第二种能够引起流行性严重脱水型霍乱的病原体，它含有一种荚膜多糖，这使得它难以用于针对霍乱弧菌O1优化的传统杀弧菌抗体检测。在对检测程序进行修改后，该程序涉及使用特定的细菌菌株、降低细菌接种量以及增加补体用量，对33例感染霍乱弧菌O139和18例感染霍乱弧菌O1的患者的急性期和恢复期血清以及20名健康对照者的单次血清样本中针对霍乱弧菌O139的杀弧菌抗体反应进行了测定。还测定了这些个体对霍乱弧菌O1菌株的反应。仅在两组霍乱患者的恢复期血清中发现同源抗体反应显著升高。这些发现可能解释了霍乱弧菌两个血清群之间缺乏异源保护的原因。健康对照者对霍乱弧菌O1的杀弧菌抗体背景水平高于对霍乱弧菌O139的。
