Veenstra J A, Lau G W, Agricola H J, Petzel D H
Department of Entomology, University of Arizona 85721, USA.
Histochem Cell Biol. 1995 Nov;104(5):337-47. doi: 10.1007/BF01458127.
The midgut of the female mosquito Aedes aegypti was studied immunohistologically with antisera to various regulatory peptides. Endocrine cells immunoreactive with antisera to perisulfakinin, RFamide, bovine pancreatic polypeptide, urotensin 1, locustatachykinin 2 and allatostatins A1 and B2 were found in the midgut. Perisulfakinin, RFamide and bovine pancreatic polypeptide all react with the same, about 500 endocrine cells, which were evenly distributed throughout the posterior midgut, with the exception of its most frontal and caudal regions. In addition, these antisera recognized three to five neurons in each ingluvial ganglion and their axons, which ran longitudinally over the anterior midgut, as well as axons innervating the pyloric sphincter. The latter axons appear to be derived from neurons located in the abdominal ganglia. Antisera to two different allatostatins recognized about 70 endocrine cells in the most caudal area of the posterior midgut and axons in the anterior midgut whose cell bodies were probably located in either the brain or the frontal ganglion. Antiserum to locustatachykinin 2 recognized endocrine cells present in the anterior midgut and the most frontal part of the posterior midgut, as well as about 50 cells in the most caudal region of the posterior midgut. Urotensin 1 immunoreactivity was found in endocrine cells in the same region as the perisulfakinin-immunoreactive cells, but no urotensin-immunoreactive axons were found in the midgut. Double labeling experiments showed that the urotensin and perisulfakinin immunoreactivities were located in different cells. Such experiments also showed that the locustatachykinin and allatostatin immunoreactivities in the most caudal area of the posterior midgut were present in different cells. No immunoreactivity was found in the mosquito midgut when using antisera to corazonin, allatropin or leucokinin IV. Since these peptides have either been isolated from, or can reasonably be expected to be present in mosquitoes, it was concluded that these peptides are not present in the mosquito midgut.
用针对各种调节肽的抗血清对埃及伊蚊雌蚊的中肠进行了免疫组织学研究。在中肠中发现了与抗硫化周肽、RF酰胺、牛胰多肽、尾加压素1、蝗虫速激肽2以及抑咽侧体素A1和B2抗血清发生免疫反应的内分泌细胞。硫化周肽、RF酰胺和牛胰多肽均与大约500个相同的内分泌细胞发生反应，这些细胞均匀分布在后肠中部，其最前端和最尾端区域除外。此外，这些抗血清在每个嗉囊神经节及其轴突中识别出三到五个神经元，这些轴突纵向穿过中肠前部，以及支配幽门括约肌的轴突。后一种轴突似乎源自位于腹神经节的神经元。针对两种不同抑咽侧体素的抗血清在后肠最尾端区域识别出约70个内分泌细胞以及中肠前部的轴突，其细胞体可能位于脑或额神经节中。针对蝗虫速激肽2的抗血清识别出存在于中肠前部和后肠最前端部分的内分泌细胞，以及后肠最尾端区域的约50个细胞。在与硫化周肽免疫反应性细胞相同的区域的内分泌细胞中发现了尾加压素1免疫反应性，但在中肠中未发现尾加压素免疫反应性轴突。双重标记实验表明，尾加压素和硫化周肽免疫反应性位于不同的细胞中。此类实验还表明，后肠最尾端区域的蝗虫速激肽和抑咽侧体素免疫反应性存在于不同的细胞中。当使用针对促心肽、咽侧体活化素或亮氨酸激肽IV的抗血清时，在蚊中肠中未发现免疫反应性。由于这些肽要么已从蚊子中分离出来，要么可以合理预期存在于蚊子中，因此得出结论，这些肽不存在于蚊中肠中。
