Departments of Biological Chemistry and Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, USA.
Mol Cell Neurosci. 1992 Aug;3(4):342-7. doi: 10.1016/1044-7431(92)90031-v.
The Drosophila drosulfakinin (dsk) gene encodes the cholecystokinin homologues drosulfakinin-I (DSK-I) and drosulfakinin-II (DSK-II). The naturally occurring DSKI peptide was isolated from an extract of adult flies and its sequence determined by automated Edman degradation and sequence-specific radioimmunoassay. The dsk cDNA is expressed during the larval, pupal, and adult stages of development and is an abundant adult head transcript. Sequence-specific DSK antibodies localized DSK expression in the Drosophila larval central nervous system to medial neurosecretory cells and projections that extend from the neurons anteriorly into the brain and posteriorly down the ventral ganglion. The availability of the dsk transcript, sequence-specific DSK antibodies and the application of molecular genetics provide the opportunity to elucidate the role(s) of Drosophila CCK homologues in brain structure and function.
果蝇的 drosulfakinin (dsk) 基因编码胆囊收缩素同源物 drosulfakinin-I (DSK-I) 和 drosulfakinin-II (DSK-II)。从成年果蝇的提取物中分离出天然存在的 DSKI 肽，并通过自动 Edman 降解和序列特异性放射免疫测定法确定其序列。dsk cDNA 在幼虫、蛹和成虫发育阶段表达，是丰富的成虫头部转录本。序列特异性 DSK 抗体将 DSK 在果蝇幼虫中枢神经系统中的表达定位到内侧神经分泌细胞和从前部延伸到大脑并向后延伸到腹神经节的投射。dsk 转录本、序列特异性 DSK 抗体的可用性以及分子遗传学的应用为阐明果蝇 CCK 同源物在大脑结构和功能中的作用提供了机会。
