Brocca S, Grandori R, Breviario D, Lotti M
Dipartimento di Fisiologia e Biochimica Generali, Italia.
Curr Genet. 1995 Oct;28(5):454-7. doi: 10.1007/BF00310815.
In the yeast Candida rugosa the lipase isozymes are encoded by a family of genes, five of which have been cloned and sequenced in our laboratory. In this paper we report on the identification and preliminary characterization of two new related sequences, thus extending this multigene family to seven members. The total DNA content of Candida cells was estimated by laser flow-cytometry at about 20 Mb. Eight chromosomes with sizes ranging between 100 kb and 2.1 Mb, as determined by comparison with S. cerevisiae chromosomal bands, were resolved by pulsed-field gel electrophoresis. The lipase-encoding genes were localized on chromosome I, therefore suggesting that they have originated through multiple duplication events of an ancestral gene.
在皱褶假丝酵母中，脂肪酶同工酶由一个基因家族编码，其中五个已在我们实验室克隆并测序。本文我们报道了两个新的相关序列的鉴定和初步表征，从而将这个多基因家族扩展到七个成员。通过激光流式细胞术估计假丝酵母细胞的总DNA含量约为20 Mb。通过与酿酒酵母染色体条带比较确定，大小在100 kb至2.1 Mb之间的八条染色体通过脉冲场凝胶电泳得以分辨。脂肪酶编码基因定位于染色体I上，因此表明它们是通过一个祖先基因的多次重复事件产生的。
