Xu Q, Alldus G, Holder N, Wilkinson D G
Developmental Biology Research Centre, Randall Institute, Kings College, London, UK.
Development. 1995 Dec;121(12):4005-16. doi: 10.1242/dev.121.12.4005.
During development of the vertebrate hindbrain regulatory gene expression is confined to precise segmental domains. Studies of cell lineage and gene expression suggest that establishment of these domains may involve a dynamic regulation of cell identity and restriction of cell movement between segments. We have taken a dominant negative approach to interfere with the function of Sek-1, a member of the Eph-related receptor tyrosine kinase family expressed in rhombomeres r3 and r5. In Xenopus and zebrafish embryos expressing truncated Sek-1, lacking kinase sequences, expression of r3/r5 markers occurs in adjacent even-numbered rhombomeres, in domains contiguous with r3 or r5. This disruption is rescued by full-length Sek-1, indicating a requirement for the kinase domain in the segmental restriction of gene expression. These data suggest that Sek-1, perhaps with other Eph-related receptors, is required for interactions that regulate the segmental identity or movement of cells.
在脊椎动物后脑发育过程中，调节基因的表达局限于精确的节段区域。细胞谱系和基因表达研究表明，这些区域的建立可能涉及细胞身份的动态调节以及节段间细胞运动的限制。我们采用了显性负性方法来干扰Sek-1的功能，Sek-1是Eph相关受体酪氨酸激酶家族的成员，在菱脑节r3和r5中表达。在表达截短的、缺乏激酶序列的Sek-1的非洲爪蟾和斑马鱼胚胎中，r3/r5标记物的表达出现在相邻的偶数菱脑节中，位于与r3或r5相邻的区域。全长Sek-1可挽救这种破坏，表明基因表达的节段性限制需要激酶结构域。这些数据表明，Sek-1可能与其他Eph相关受体一起，是调节细胞节段身份或运动的相互作用所必需的。
