Sauvant M P, Pepin D, Bohatier J, Groliere C A
Faculté de Pharmacie, Laboratoire Hydrologie & Hygiène, Clermont-Ferrand, France.
Ecotoxicol Environ Saf. 1995 Nov;32(2):159-65. doi: 10.1006/eesa.1995.1097.
The microplate technique (MT) is developed and compared to the usual flask technique (FT) of Tetrahymena pyriformis GL cultures for assessing toxic effects of organic and inorganic substances on this ciliated protozoa model. Applied to the IC50 determination of 29 xenobiotics, the MT demonstrated good correlation with the FT (Y = 0.991X + 0.012, r = 0.94, P < 0.0001). The MT is rapid, easy-to-handle, inexpensive, and statistically reliable and, so, may be helpful for screening investigations of water-soluble xenobiotics.
开发了微孔板技术（MT），并将其与梨形四膜虫GL培养物的常规烧瓶技术（FT）进行比较，以评估有机和无机物质对这种纤毛原生动物模型的毒性作用。将MT应用于29种外源化合物的IC50测定，结果表明MT与FT具有良好的相关性（Y = 0.991X + 0.012，r = 0.94，P < 0.0001）。MT快速、易于操作、成本低廉且统计可靠，因此可能有助于水溶性外源化合物的筛选研究。
