Ronéus M, Persson S G, Essén-Gustavsson B, Arnason T
Department of Medicine and Surgery, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Equine Vet J. 1994 Jul;26(4):319-22. doi: 10.1111/j.2042-3306.1994.tb04393.x.
Muscle biopsy specimens from the middle gluteal muscle were studied in 16 red blood cell hypervolaemic (Group HV) and 19 normovolaemic (Group NV) Standardbred racehorses. All horses were stallions, 4-8 years old and having similar mean racing performance values, as described by an individual selection index value. All horses raced regularly but those in Group HV did not perform as expected and were therefore referred to the clinics for exercise tolerance testing. Muscle biopsy specimens were analysed for fibre type distribution (Type I, IIA and IIB), fibre area and relative fibre area. In addition, oxidative capacity of the fibres was evaluated by staining for nicotinamide adenine dinucleotide (NADH) tetrazolium reductase, and the activities of citrate synthase, 3-OH-acyl CoA dehydrogenase and lactate dehydrogenase were analysed in whole-muscle samples. With the exception of a higher percentage of Type IIB fibres in Group HV having a high oxidative capacity as evaluated by the NADH stain, no significant difference were found in fibre composition, fibre area or enzyme activity between the Groups HV and NV.
对16匹红细胞血容量过多（HV组）和19匹血容量正常（NV组）的标准赛马的臀中肌肌肉活检标本进行了研究。所有马匹均为4 - 8岁的雄性马，且根据个体选择指数值，它们的平均比赛成绩相近。所有马匹都定期参赛，但HV组的马匹表现未达预期，因此被转诊至诊所进行运动耐力测试。对肌肉活检标本进行纤维类型分布（I型、IIA型和IIB型）、纤维面积和相对纤维面积分析。此外，通过烟酰胺腺嘌呤二核苷酸（NADH）四唑还原酶染色评估纤维的氧化能力，并在全肌肉样本中分析柠檬酸合酶、3 - 羟基酰基辅酶A脱氢酶和乳酸脱氢酶的活性。除了通过NADH染色评估发现HV组中具有高氧化能力的IIB型纤维百分比更高外，HV组和NV组在纤维组成、纤维面积或酶活性方面未发现显著差异。
