Xu H, Wang C, Zhu H, Liu S, Xu X, Jiang Y
Department of Ophthalmology, Xiangya Hospital, Hunan Medical University, Changsha, China.
Yan Ke Xue Bao. 1995 Mar;11(1):16-21.
To study the retinoblastoma cell culture and to establish a new retinoblastoma cell line.
22 retinoblastomas were cultured by using the method of single cell suspension. Characteristics of the cultured cells were studied in the following programs: tumor cell morphology in vitro, electron microscopic, growth curve, cloning in soft agar, immunohistochemistry, karyotype and tumorigenicity.
22 retinoblastomas were cultured successfully in vitro, only a continued cell line HXO-Rb44 was established (more than 3 years). The characteristics of this cell line are that it grew as a suspension of round cell in graps like clusters in vitro, its population doubling time was 44 hours, and it could be cloned in soft agar. Histopathologic and ultrastructured pictures showed the characteristics of Rb. HXO-Rb44 cell was positive to NSE and negative to GFAP in immunohistochemical staining. A subcutaneous injection of HXO-Rb44 cells produced a retinoblastoma in BALB/C athumic nude mice.
HXO-Rb44 has the characteristics of retinoblastoma and is a new retinoblastoma cell line. It is a useful material for study this tumor both in basic and clinical fields.
研究视网膜母细胞瘤细胞培养并建立一种新的视网膜母细胞瘤细胞系。
采用单细胞悬液法培养22例视网膜母细胞瘤。通过以下项目研究培养细胞的特性：体外肿瘤细胞形态、电子显微镜观察、生长曲线、软琼脂克隆、免疫组织化学、核型分析及致瘤性。
22例视网膜母细胞瘤成功进行了体外培养，仅建立了一个连续传代细胞系HXO-Rb44（传代超过3年）。该细胞系的特点是在体外呈圆形细胞悬液生长，聚集成葡萄串状，群体倍增时间为44小时，且能在软琼脂中克隆。组织病理学和超微结构图像显示具有视网膜母细胞瘤的特征。免疫组织化学染色显示HXO-Rb44细胞对神经元特异性烯醇化酶（NSE）呈阳性反应，对胶质纤维酸性蛋白（GFAP）呈阴性反应。将HXO-Rb44细胞皮下注射到BALB/C无胸腺裸鼠体内可产生视网膜母细胞瘤。
HXO-Rb44具有视网膜母细胞瘤的特征，是一种新的视网膜母细胞瘤细胞系。它是基础和临床领域研究该肿瘤的有用材料。
