Dubash K, Shulaw W P, Bech-Nielsen S, Stills H F, Slemons R D
Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Ohio State University, Columbus 43210, USA.
J Am Vet Med Assoc. 1996 Feb 1;208(3):401-3.
To determine whether a commercially available agar gel immunodiffusion test approved for detecting antibodies to Mycobacterium paratuberculosis in cattle could be used for sheep.
Experimental trial.
Serum samples from 27 sheep confirmed to have paratuberculosis by means of acid-fast staining of smears of ileal mucosa, histologic examination of tissues, or bacteriologic culture; 7 sheep with clinical signs of paratuberculosis; and 55 sheep from 5 uninfected flocks.
Serum samples were tested concurrently with the commercially available test and with a previously validated agar gel immunodiffusion test. Multiple samples collected from 13 infected sheep over a period of 6 years were also tested so that each test's ability to detect onset of seropositivity could be compared.
For both tests, results for samples from all 55 uninfected sheep were negative, results for samples from 32 of the 34 sheep with paratuberculosis were positive, and results for the remaining 2 sheep with paratuberculosis were negative. Results of both tests were in agreement for 50 of 54 samples obtained from 13 infected sheep over time. The 4 samples for which results of the 2 tests disagreed were the fourth, eighth, and ninth of 10 samples from 1 sheep and the first of 6 samples from a second sheep. For all 4 samples, the commercially available assay yielded a weak-positive result, but the previously described test yielded a negative result.
The commercially available agar gel immunodiffusion test approved for use in cattle may be useful in the differential diagnosis of paratuberculosis in sheep.
确定一种已获批准用于检测牛副结核分枝杆菌抗体的市售琼脂凝胶免疫扩散试验是否可用于绵羊。
实验性试验。
通过回肠黏膜涂片抗酸染色、组织学检查或细菌培养确诊患有副结核病的27只绵羊的血清样本；7只具有副结核病临床症状的绵羊；以及来自5个未感染羊群的55只绵羊。
血清样本同时用市售试验和先前验证过的琼脂凝胶免疫扩散试验进行检测。还对13只感染绵羊在6年期间采集的多个样本进行了检测，以便比较每种试验检测血清阳性起始的能力。
对于两种试验，所有55只未感染绵羊的样本结果均为阴性，34只患有副结核病绵羊中的32只样本结果为阳性，其余2只患有副结核病绵羊的样本结果为阴性。随着时间推移，从13只感染绵羊获得的54个样本中，有50个样本的两种试验结果一致。两种试验结果不一致的4个样本分别是来自1只绵羊的10个样本中的第4、第8和第9个样本以及来自另一只绵羊的6个样本中的第1个样本。对于所有4个样本，市售检测产生弱阳性结果，但先前描述的试验产生阴性结果。
已获批准用于牛的市售琼脂凝胶免疫扩散试验可能有助于绵羊副结核病的鉴别诊断。
