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通过逆转录依赖性聚合酶链反应对鱼类致病性弹状病毒进行鉴别诊断。

Differential diagnosis of fish pathogenic rhabdoviruses by reverse transcriptase-dependent polymerase chain reaction.

作者信息

Bruchhof B, Marquardt O, Enzmann P J

机构信息

Federal Research Centre for Virus Diseases of Animals, Tübingen, Germany.

出版信息

J Virol Methods. 1995 Sep;55(1):111-9. doi: 10.1016/0166-0934(95)00051-u.

DOI:10.1016/0166-0934(95)00051-u
PMID:8576301
Abstract

Reverse transcriptase-dependent polymerase chain reaction (RT-PCR) was applied to the detection and differentiation of viral hemorrhagic septicemia virus (VHSV) and infectious hematopoietic necrosis virus (IHNV) using primer pairs designed for the amplification of glycoprotein G-specific gene fragments of the two viruses. The products of 443 bp (VHS) and 548 bp (IHN), respectively, were amplified from the total RNA extracts of RTG-2 cells infected with a total of 9 different strains of either VHS virus or IHN virus. Restriction analysis using FokI, and DNA sequencing of the PCR products demonstrated specificity of the amplification. The RT-PCR amplification of VHSV or IHNV G-genes was found to be a simple, highly specific and sensitive method allowing differential diagnosis of VHS and IHN within 8 h.

摘要

采用逆转录酶依赖性聚合酶链反应(RT-PCR),利用为扩增两种病毒糖蛋白G特异性基因片段而设计的引物对,对病毒性出血性败血症病毒(VHSV)和传染性造血器官坏死病毒(IHNV)进行检测和鉴别。分别从感染了总共9种不同株VHS病毒或IHNV病毒的RTG-2细胞的总RNA提取物中扩增出443 bp(VHS)和548 bp(IHN)的产物。使用FokI进行限制性分析以及对PCR产物进行DNA测序,证明了扩增的特异性。发现VHSV或IHNV G基因的RT-PCR扩增是一种简单、高度特异且灵敏的方法,能够在8小时内对VHS和IHN进行鉴别诊断。

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Differential diagnosis of fish pathogenic rhabdoviruses by reverse transcriptase-dependent polymerase chain reaction.通过逆转录依赖性聚合酶链反应对鱼类致病性弹状病毒进行鉴别诊断。
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引用本文的文献

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"Mid-G" region sequences of the glycoprotein gene of Austrian infectious hematopoietic necrosis virus isolates form two lineages within European isolates and are distinct from American and Asian lineages.奥地利传染性造血坏死病毒分离株糖蛋白基因的“Mid-G”区域序列在欧洲分离株中形成两个谱系,且与美洲和亚洲谱系不同。
J Clin Microbiol. 2008 Jan;46(1):22-30. doi: 10.1128/JCM.00566-07. Epub 2007 Oct 31.
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Reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus (VHS).用于快速检测病毒性出血性败血症病毒(VHS)的逆转录环介导等温扩增技术(RT-LAMP)
Vet Microbiol. 2006 May 31;114(3-4):205-13. doi: 10.1016/j.vetmic.2005.11.063. Epub 2005 Dec 27.
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Multiplex reverse transcriptase PCR assay for simultaneous detection of three fish viruses.
用于同时检测三种鱼类病毒的多重逆转录聚合酶链式反应检测法
J Clin Microbiol. 1999 Dec;37(12):4139-41. doi: 10.1128/JCM.37.12.4139-4141.1999.