Berny P J, Buronfosse T, Lorgue G
Département des Sciences Pharmaceutiques et Toxicologiques, Ecole Nationale Vétérinaire de Lyon, Marcy l'Etoile, France.
J Anal Toxicol. 1995 Nov-Dec;19(7):576-80. doi: 10.1093/jat/19.7.576.
The purpose of this study was to develop and evaluate a technique for the analysis of anticoagulant rodenticides in serum and liver samples using a new high-performance thin-layer chromatographic apparatus. Detection limits were estimated at 0.2 micrograms/g in liver extracts for eight different substances. Overall, this technique was repeatable and reproducible. The percent recovery was greater than 87% for each substance. Liver and serum samples of animals known to be exposed to one anticoagulant and showing clinical signs of poisoning were analyzed. Concentrations measured varied between 0.2 and 3 micrograms/g (liver extracts). Only blood samples from one dog could be analyzed. The concentration was 150 ng/mL the first day after admission and 140 ng/mL the following day. Analyses are technically easily and rapidly performed, and they are inexpensive. Therefore, this technique could be a valuable alternative to current high-performance liquid chromatographic methods.
本研究的目的是开发并评估一种使用新型高效薄层色谱仪分析血清和肝脏样本中抗凝血灭鼠剂的技术。对八种不同物质的肝脏提取物检测限估计为0.2微克/克。总体而言，该技术具有可重复性和再现性。每种物质的回收率均大于87%。对已知接触过一种抗凝血剂并出现中毒临床症状的动物的肝脏和血清样本进行了分析。测得的浓度在0.2至3微克/克（肝脏提取物）之间变化。仅对一只狗的血样进行了分析。入院第一天浓度为150纳克/毫升，第二天为140纳克/毫升。分析在技术上易于快速进行，且成本低廉。因此，该技术可能是当前高效液相色谱法的一种有价值的替代方法。
