Norris V
Department of Microbiology and Immunology, School of Medicine, University of Leicester, UK.
Mol Microbiol. 1995 Jun;16(6):1051-7. doi: 10.1111/j.1365-2958.1995.tb02330.x.
To explain how daughter chromosomes are separated into discrete nucleoids and why chromosomes are partitioned with pole preferences, I propose that differential gene expression occurs during DNA replication in Escherichia coli. This differential gene expression means that the daughter chromosomes have different patterns of gene expression and that cell division is not a simple process of binary fission. Differential gene expression arises from autocatalytic gene expression and creates a separate proteolipid domain around each developing chromosome via the coupled transcription-translation-insertion of proteins into membranes (transertion). As these domains are immiscible, daughter chromosomes are simultaneously replicated and separated into discrete nucleoids. I also propose that the partitioning relationship between chromosome age and cell age arises because the poles of cells have a proteolipid composition that favours transertion from one nucleoid rather than from the other. This hypothesis forms part of an ensemble of related hypotheses which attempt to explain cell division, differentiation and wall growth in bacteria in terms of the physical properties and interactions of the principal constituents of cells.
为了解释子代染色体如何被分离到离散的类核中，以及为什么染色体以极性偏好进行分配，我提出在大肠杆菌的DNA复制过程中会发生差异基因表达。这种差异基因表达意味着子代染色体具有不同的基因表达模式，并且细胞分裂不是简单的二分分裂过程。差异基因表达源于自催化基因表达，并通过蛋白质的转录 - 翻译 - 插入到膜中（转插入）在每个发育中的染色体周围创建一个单独的蛋白脂质结构域。由于这些结构域互不相溶，子代染色体同时被复制并分离到离散的类核中。我还提出染色体年龄与细胞年龄之间的分配关系的产生是因为细胞的两极具有有利于从一个类核而非另一个类核进行转插入的蛋白脂质组成。这个假设构成了一系列相关假设的一部分，这些假设试图根据细胞主要成分的物理性质和相互作用来解释细菌中的细胞分裂、分化和细胞壁生长。
