Kozaki S, Nakaue S, Kamata Y
Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan.
Microbiol Immunol. 1995;39(10):767-74. doi: 10.1111/j.1348-0421.1995.tb03269.x.
The neurotoxin associated with type A infant botulism in Japan shows different antigenic properties from those produced by authentic strains. The monoclonal antibodies recognizing the light chain reacted to both neurotoxins, whereas half the antibodies recognizing the heavy chain reacted specifically to the respective neurotoxin. Each neurotoxin showed its own manner of binding to brain synaptosomes. These results indicate that the distinguishable characteristics are ascribable to the heavy chain but not to the light chain. In both neurotoxins, an epitope recognized by the monoclonal antibody that reacts to the light chain and neutralizes the toxin was found to be very close to the amino-terminal half (H-1 fragment) of the heavy chain. This may support the hypothesis that the H-1 fragment functions in the transport of the light chain in the target cell.
在日本，与A型婴儿肉毒中毒相关的神经毒素显示出与正宗菌株产生的神经毒素不同的抗原特性。识别轻链的单克隆抗体与两种神经毒素都发生反应，而识别重链的抗体中有一半特异性地与各自的神经毒素发生反应。每种神经毒素都有其自身与脑突触体结合的方式。这些结果表明，可区分的特征归因于重链而非轻链。在两种神经毒素中，发现与轻链反应并中和毒素的单克隆抗体识别的一个表位非常靠近重链的氨基末端半段（H-1片段）。这可能支持H-1片段在靶细胞中轻链运输中起作用的假说。
