Ho T, Htwe K K, Yamasaki N, Zhang G Q, Ogawa M, Yamaguchi T, Fukushi H, Hirai K
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
Microbiol Immunol. 1995;39(9):663-71. doi: 10.1111/j.1348-0421.1995.tb03254.x.
Coxiella burnetii was isolated from raw milk (36/214, 16.8%) and uterus swab samples (13/61, 21.3%) originating from dairy cattle with reproductive disorders, aborted bovine fetus samples (2/4, 50%), mammary gland samples (4/50, 8%) originating from healthy dairy cattle, and tick samples (4/15, 26.7%) originating from 2 pastures. Fifty-nine strains had various degrees of pathogenicity, high (8; 13.6%), moderate (28; 47.5%) and low (23; 39%), for guinea pigs. The results of isolation suggested a high prevalence of Coxiella infection in dairy cattle with reproductive problems in Japan. Twelve strains (7, 2 and 3 strains from cattle, ticks and humans, respectively) and the reference Nine Mile strain of phases I and II were propagated in both yolk sacs of embryonated hen eggs and Buffalo green monkey (BGM) cell cultures. Protein profiles of these strains were similar to those of the reference strain of phase I. Lipopolysaccharide (LPS) profiles of 12 strains were similar to those of the reference strain of phase I and different from those of the reference strain of phase II. The LPS profiles of 12 strains suggested that these strains are associated with an acute form of Q fever.
从患有生殖障碍的奶牛的生乳（36/214，16.8%）和子宫拭子样本（13/61，21.3%）、流产的牛胎儿样本（2/4，50%）、来自健康奶牛的乳腺样本（4/50，8%）以及来自2个牧场的蜱样本（4/15，26.7%）中分离出了伯氏考克斯体。59株菌株对豚鼠具有不同程度的致病性，高致病性（8株；13.6%）、中度致病性（28株；47.5%）和低致病性（23株；39%）。分离结果表明，日本患有生殖问题的奶牛中伯氏考克斯体感染率很高。12株菌株（分别来自牛、蜱和人的7株、2株和3株）以及I期和II期的参考九里菌株在鸡胚卵黄囊和水牛绿猴（BGM）细胞培养物中均进行了增殖培养。这些菌株的蛋白质谱与I期参考菌株的相似。12株菌株的脂多糖（LPS）谱与I期参考菌株的相似，与II期参考菌株的不同。12株菌株的LPS谱表明这些菌株与急性Q热有关。
