患有生殖系统疾病的奶牛中伯氏考克斯氏体感染的患病率
Prevalence of Coxiella burnetii infection in dairy cattle with reproductive disorders.
作者信息
To H, Htwe K K, Kako N, Kim H J, Yamaguchi T, Fukushi H, Hirai K
机构信息
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
出版信息
J Vet Med Sci. 1998 Jul;60(7):859-61. doi: 10.1292/jvms.60.859.
The prevalence of Coxiella burnetii infection in 207 cattle with reproductive disorders was studied by using an indirect immunofluorescence (IF) test, nested polymerase chain reaction (PCR) and isolation. IF antibodies to phase I and phase II antigens of C. burnetii were found in 122 (58.9%) and 125 (60.4%) of the sera, respectively, and PCR-positives were found in 8 (3.9%) of the sera and in 51 (24.6%) of the milk samples. In addition, C. burnetii was isolated from 51 (24.6%) of the milk samples by inoculating laboratory mice. The results indicate that the IF test plus PCR are useful in the diagnosis of bovine coxiellosis. It is difficult to deny that dairy cattle with reproductive disorders would be one of the important reservoirs of C. burnetii responsible for infection in both animal and human populations in Japan.
通过间接免疫荧光（IF）试验、巢式聚合酶链反应（PCR）和分离培养，对207头患有生殖障碍的奶牛进行了伯氏考克斯体感染率的研究。分别在122份（58.9%）和125份（60.4%）血清中检测到针对伯氏考克斯体I相和II相抗原的IF抗体，在8份（3.9%）血清和51份（24.6%）乳样中检测到PCR阳性。此外，通过接种实验小鼠，从51份（24.6%）乳样中分离出伯氏考克斯体。结果表明，IF试验加PCR对牛考克斯体病的诊断有用。很难否认，患有生殖障碍的奶牛可能是日本动物和人类群体感染伯氏考克斯体的重要储存宿主之一。
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