Murase T, Okitsu T, Suzuki R, Morozumi H, Matsushima A, Nakamura A, Yamai S
Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Japan.
Microbiol Immunol. 1995;39(9):673-6. doi: 10.1111/j.1348-0421.1995.tb03255.x.
To evaluate DNA fingerprinting as an epidemiologic tool, pulsed-field gel electrophoresis (PFGE) was performed on isolates of Salmonella, including S. typhimurium, S. thompson, and S. enteritidis. Chromosomal DNA was digested with the restriction endonucleases Bln I and Xba I. The patterns of S. thompson and S. typhimurium isolates from various sources were different from one another. There was no correlation between the phage type and the digestion pattern of S. enteritidis isolates. Some strains belonging to one phage type were distinguished by their PFGE pattern in this study. These results suggest that the Bln I and Xba I digestion patterns of chromosomal DNA are useful for epidemiological analysis of an outbreak of Salmonella infection or food poisoning.
为评估DNA指纹图谱作为一种流行病学工具的作用，对包括鼠伤寒沙门氏菌、汤普森沙门氏菌和肠炎沙门氏菌在内的沙门氏菌分离株进行了脉冲场凝胶电泳（PFGE）分析。用限制性内切酶Bln I和Xba I消化染色体DNA。来自不同来源的汤普森沙门氏菌和鼠伤寒沙门氏菌分离株的图谱彼此不同。肠炎沙门氏菌分离株的噬菌体类型与消化图谱之间没有相关性。在本研究中，一些属于同一噬菌体类型的菌株通过其PFGE图谱得以区分。这些结果表明，染色体DNA的Bln I和Xba I消化图谱可用于沙门氏菌感染暴发或食物中毒的流行病学分析。
