Lyall F, Greer I A, Boswell F, Young A, Macara L M, Jeffers M D
Department of Obstetrics and Gynaecology, University of Glasgow, Royal Infirmary, UK.
Placenta. 1995 Oct;16(7):579-87. doi: 10.1016/0143-4004(95)90027-6.
Platelets and neutrophils are involved in maternal placental vascular damage in pre-eclampsia. Recruitment of these cells is probably mediated by cell adhesion molecules expressed at the uteroplacental bed. It remains controversial as to whether platelets and neutrophils mediate damage to trophoblast or villous vasculature. The purpose of this study was to determine the expression of cell adhesion molecules in placentae from normal pregnancies and pregnancies complicated by pre-eclampsia and intrauterine growth retardation (IUGR). Immunostaining for platelet endothelial cell adhesion molecule (PECAM) and intercellular adhesion molecule-1 (ICAM-1) was localized mainly to the endothelium of stem villi, intermediate villi, terminal villi and decidual vessels. Scattered staining for ICAM-1 was also evident in the stroma and fetal membranes. The endothelium of stem villi, intermediate villi and terminal villi were all negative for vascular cell adhesion molecule-1 (VCAM-1) and E-Selectin. PECAM, ICAM-1 and ICAM-2 mRNA were all detectable in normal placentae using northern blotting analysis whereas mRNA for E-Selectin and VCAM-1 were both undetectable. There were no differences in cell adhesion molecule immunostaining or mRNA expression in placentae from pregnancies complicated by pre-eclampsia and IUGR inconclusion, expression of cell adhesion molecules in placentae from pre-eclampsia and IUGR are consistent with a normal physiological role in vascular function.
血小板和中性粒细胞参与子痫前期孕妇胎盘血管损伤。这些细胞的募集可能由子宫胎盘床表达的细胞黏附分子介导。血小板和中性粒细胞是否介导对滋养层或绒毛血管的损伤仍存在争议。本研究的目的是确定正常妊娠及合并子痫前期和宫内生长受限（IUGR）妊娠的胎盘组织中细胞黏附分子的表达情况。血小板内皮细胞黏附分子（PECAM）和细胞间黏附分子-1（ICAM-1）的免疫染色主要定位于主干绒毛、中间绒毛、终末绒毛和蜕膜血管的内皮。ICAM-1在基质和胎膜中也有散在染色。主干绒毛、中间绒毛和终末绒毛的内皮对血管细胞黏附分子-1（VCAM-1）和E-选择素均呈阴性。采用Northern印迹分析在正常胎盘中均可检测到PECAM、ICAM-1和ICAM-2的mRNA，而E-选择素和VCAM-1的mRNA均未检测到。子痫前期和IUGR妊娠胎盘的细胞黏附分子免疫染色或mRNA表达无差异。总之，子痫前期和IUGR胎盘组织中细胞黏附分子的表达与血管功能的正常生理作用一致。
