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基因控制区的研究。2. 化学合成的乳糖操纵子脱氧核糖核酸片段的酶促连接。

Studies on gene control regions. 2. Enzymatic joining of chemically synthesized lactose operator deoxyribonucleic acid segments.

作者信息

Yansura D G, Goeddel D V, Caruthers M H

出版信息

Biochemistry. 1977 May 3;16(9):1772-80. doi: 10.1021/bi00628a002.

DOI:10.1021/bi00628a002
PMID:857879
Abstract

The T4 polynucleotide ligase catalyzed joining of six chemically synthesized deoxypolynucleotides corresponding to lactose operator DNA has been investigated. Joining was studied using various combinations of segments. Joining reactions involving multiple sites and the formation of duplex operator DNA were complete in a few hours. Joining reactions involving a single site and the formation of only one strand of operator DNA required several days and repeated annealing in order to go to completion. These studies have permitted the synthesis on a preparative scale (several nanomoles) of operator duplexes and operator single strands.

摘要

对T4多核苷酸连接酶催化的六个对应于乳糖操纵基因DNA的化学合成脱氧多核苷酸的连接进行了研究。使用片段的各种组合研究连接情况。涉及多个位点和双链操纵基因DNA形成的连接反应在几小时内完成。涉及单个位点和仅一条操纵基因DNA链形成的连接反应需要几天时间并反复退火才能完成。这些研究使得能够以制备规模（几纳摩尔）合成操纵基因双链体和操纵基因单链。

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Studies on gene control regions. 1. Chemical synthesis of lactose operator deoxyribonucleic acid segments.基因控制区的研究。1. 乳糖操纵基因脱氧核糖核酸片段的化学合成。
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引用本文的文献

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Binding studies of SV40 T-antigen to SV40 binding site II.猴空泡病毒40 T抗原与猴空泡病毒40结合位点II的结合研究。
Nucleic Acids Res. 1985 Sep 25;13(18):6621-34. doi: 10.1093/nar/13.18.6621.
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DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels.
DNA链长标记物以及碱基组成对寡脱氧核糖核苷酸在聚丙烯酰胺凝胶中电泳迁移率的影响。
Nucleic Acids Res. 1979;6(6):2069-87. doi: 10.1093/nar/6.6.2069.
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Studies on gene control regions XII. The functional significance of a lac operator constitutive mutation.基因调控区域的研究十二。乳糖操纵子组成型突变的功能意义。
Nucleic Acids Res. 1979 Sep 25;7(2):401-16. doi: 10.1093/nar/7.2.401.
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Studies on gene control regions X. The effect of specific adenine-thymine transversions on the lac repressor-lac operator interaction.基因控制区的研究X. 特定腺嘌呤-胸腺嘧啶颠换对乳糖阻遏物-乳糖操纵子相互作用的影响。
Nucleic Acids Res. 1979 Jun 11;6(7):2583-99. doi: 10.1093/nar/6.7.2583.
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Proc Natl Acad Sci U S A. 1977 Aug;74(8):3292-6. doi: 10.1073/pnas.74.8.3292.
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