大肠杆菌乳糖操纵基因的化学与酶促合成及其与乳糖阻遏物的结合
Chemical and enzymatic synthesis of lactose operator of Escherichia coli and its binding to lactose repressor.
作者信息
Bahl C P, Wu R, Itakura K, Katagiri N, Narang S A
出版信息
Proc Natl Acad Sci U S A. 1976 Jan;73(1):91-4. doi: 10.1073/pnas.73.1.91.
Abstract
The 21-nucleotide-long duplex DNA constituting the lactose operator sequence of E. coli has been synthesized by both chemical and enzymatic methods. The synthetic duplex has the essential feature of the lactose operator as seen by its binding to the lactose repressor. The binding of the synthetic operator fragment to the lactose repressor is specific because it is inhibited by the inducing ligand isopropyl-beta-D-thiogalactoside. Thus, it is now possible to show that a chemically synthesized oligodeoxynucleotide can be specifically recognized by its natural regulatory protein.
摘要
通过化学和酶促方法合成了构成大肠杆菌乳糖操纵序列的21个核苷酸长的双链DNA。从其与乳糖阻遏物的结合情况来看，合成的双链体具有乳糖操纵子的基本特征。合成的操纵子片段与乳糖阻遏物的结合是特异性的，因为它受到诱导配体异丙基-β-D-硫代半乳糖苷的抑制。因此，现在可以证明化学合成的寡脱氧核苷酸能够被其天然调节蛋白特异性识别。
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Chemical and enzymatic synthesis of lactose operator of Escherichia coli and its binding to lactose repressor.大肠杆菌乳糖操纵基因的化学与酶促合成及其与乳糖阻遏物的结合
Proc Natl Acad Sci U S A. 1976 Jan;73(1):91-4. doi: 10.1073/pnas.73.1.91.
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本文引用的文献
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