Yu Q, Shi H, Wang J
Union Hospital, Tongji Medical University, Wuhan.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1995;30(3):152-3.
A simultaneous double-staining procedure using fluorescein diacetate (FDA) and propidium iodide (PI) is discribed for use in the determination of isolated cochlear outer hair cell viability. With exciter light, viable cells fluoresce bright green, while nonviable cells are bright red. In cell culture and cytotoxicity studies, double-staining with FDA-PI is a accurate method to discriminate between live and nonviable cells.
描述了一种使用荧光素二乙酸酯（FDA）和碘化丙啶（PI）的同步双重染色程序，用于确定分离的耳蜗外毛细胞的活力。在激发光下，活细胞发出亮绿色荧光，而非活细胞则发出亮红色荧光。在细胞培养和细胞毒性研究中，FDA-PI双重染色是区分活细胞和非活细胞的一种准确方法。
