Adams A M, Soames J V, Searle R F
Arch Oral Biol. 1986;31(8):529-33. doi: 10.1016/0003-9969(86)90146-9.
Three micrograms of amphotericin B/ml nutrient medium markedly inhibited the monolayer outgrowth of cells from explants of human periodontal ligament. Amphotericin B (1.5 microgram/ml) retarded outgrowth with morphological evidence of cell injury. The addition of 3 micrograms/ml amphotericin B to existing monolayers initially caused disruption of the majority of cells but those which survived then proliferated. Thus, routine use of amphotericin B is contra-indicated in tissue culture of human periodontal ligament.
每毫升营养培养基中含三微克两性霉素B可显著抑制人牙周膜外植体的细胞单层生长。两性霉素B（每毫升1.5微克）会阻碍生长，并出现细胞损伤的形态学证据。向现有的细胞单层中添加每毫升3微克两性霉素B最初会导致大多数细胞破裂，但存活下来的细胞随后会增殖。因此，在人牙周膜组织培养中常规使用两性霉素B是禁忌的。
