大鼠食管运动前神经元中一氧化氮合酶与N-甲基-D-天冬氨酸受体的共定位

Co-localization of NOS and NMDA receptor in esophageal premotor neurons of the rat.

作者信息

Broussard D L, Bao X, Li X, Altschuler S M

机构信息

Division of Gastrenterology and Nutrition, Children's Hospital of Philadelphia, PA 19104, USA.

出版信息

Neuroreport. 1995 Oct 23;6(15):2073-6. doi: 10.1097/00001756-199510010-00028.

DOI:10.1097/00001756-199510010-00028

PMID:8580443

Abstract

Nitric oxide (NO) production following NMDA receptor stimulation plays a role in signaling between neurons. Using trans-synaptic tracing with pseudorabies virus (PRV), immunocytochemistry and histochemistry, we have demonstrated the expression of NMDAR1 and nitric oxide synthase (NOS) within brain stem neurons controlling esophageal peristalsis. PRV-immunoreactive second order esophageal premotor neurons of the central subnucleus of the nucleus of the solitary (NTScen) expressed NMDAR1 and NOS. First order motoneurons of the compact formation of the nucleus ambiguus (NAc) expressed NMDAR1, but did not contain NOS. NTScen neurons may synthesize and release NO in response to NMDA activation, suggesting a role for NO in the coordination of esophageal motility.

摘要

N-甲基-D-天冬氨酸（NMDA）受体刺激后一氧化氮（NO）的产生在神经元之间的信号传导中起作用。通过使用伪狂犬病病毒（PRV）进行跨突触追踪、免疫细胞化学和组织化学，我们已经证明了在控制食管蠕动的脑干神经元中NMDAR1和一氧化氮合酶（NOS）的表达。孤束核中央亚核（NTScen）的PRV免疫反应性二级食管前运动神经元表达NMDAR1和NOS。疑核紧密部（NAc）的一级运动神经元表达NMDAR1，但不含有NOS。NTScen神经元可能会响应NMDA激活而合成并释放NO，这表明NO在食管运动协调中发挥作用。