Barrett R T, Bao X, Miselis R R, Altschuler S M
Division of Gastroenterology and Nutrition, Children's Hospital of Philadelphia, Pennsylvania.
Gastroenterology. 1994 Sep;107(3):728-37. doi: 10.1016/0016-5085(94)90120-1.
BACKGROUND/AIMS: Brain stem premotor neurons control swallowing through contacts with both afferent neurons and motoneurons. The location and connectivity of premotor neurons innervating the esophagus was determined using pseudorabies virus.
In 30 rats, viral injections were made into either the cervical or subdiaphragmatic esophagus, cricothyroid muscle, or stomach. After a 48-62-hour survival, brain sections were processed immunocytochemically for the virus.
Neuronal labeling was limited to the compact formation of the nucleus ambiguus for survivals of 48-54 hours. At 57-62-hour survivals, virus-labeled second-order neurons (premotor neurons) were localized to the central subnucleus of nucleus of the solitary tract. Injections in the cricothyroid muscle and stomach resulted in distinct patterns of motoneuronal labeling in the nucleus ambiguus and dorsal motor nucleus and premotor neuronal labeling in the nucleus of the solitary tract.
Virus-labeled premotor neurons in the nucleus of the solitary tract occurred as a result of retrograde transport of the virus from the nucleus ambiguus because no viral antigen was present in the tractus solitarius. The esophagus is controlled by a central circuit whereby esophageal vagal afferents terminate on premotor neurons in the central subnucleus that in turn innervate esophageal motoneurons in the nucleus ambiguus.
背景/目的：脑干运动前神经元通过与传入神经元和运动神经元的联系来控制吞咽。使用伪狂犬病病毒确定支配食管的运动前神经元的位置和连接性。
对30只大鼠，将病毒注射到颈段或膈下食管、环甲肌或胃中。存活48 - 62小时后，对脑切片进行病毒免疫细胞化学处理。
在存活48 - 54小时时，神经元标记仅限于疑核的致密形成区。在存活57 - 62小时时，病毒标记的二级神经元（运动前神经元）定位于孤束核的中央亚核。向环甲肌和胃注射导致疑核和背运动核中运动神经元标记以及孤束核中运动前神经元标记呈现不同模式。
孤束核中病毒标记的运动前神经元是由于病毒从疑核逆行运输所致，因为孤束中不存在病毒抗原。食管由一个中枢回路控制，食管迷走传入神经终止于中央亚核的运动前神经元，这些运动前神经元转而支配疑核中的食管运动神经元。
