Wiedner E B, Bao X, Altschuler S M
Division of Gastroenterology and Nutrition, Children's Hospital of Philadelphia, Pennsylvania.
Gastroenterology. 1995 Feb;108(2):367-75. doi: 10.1016/0016-5085(95)90062-4.
BACKGROUND/AIMS: The central subnucleus of the nucleus of the solitary tract has been implicated in central reflex control of esophageal peristalsis. This study determined the presence of nitric oxide synthase in the brain stem circuit controlling esophageal peristalsis by combining transsynaptic retrograde tract tracing with pseudorabies virus and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH) histochemistry.
Virus was injected into the esophagus of 10 of 15 rats. After 60-63 hours, brain sections were processed for viral immunofluorescence and NADPH histochemistry.
Fluorescent neuronal labeling was limited to the compact formation of the nucleus ambiguus and the central subnucleus of the nucleus of the solitary tract. Most fluorescence-labeled neurons in the central subnucleus stained positively for NADPH (double labeled). In the compact formation, there were almost no double-labeled neurons; however, NADPH-stained terminals surrounded fluorescence-labeled motoneurons.
NO synthase is present in premotor neurons of the central subnucleus of the nucleus of the solitary tract that innervate esophageal motoneurons in the compact formation of the nucleus ambiguus. NADPH staining in both somata and terminals of esophageal premotor neurons suggests that NO is involved in neurotransmission in the central subnucleus and at the site of synaptic contact between esophageal premotor neurons and motoneurons in the compact formation of the nucleus ambiguus.
背景/目的：孤束核的中央亚核与食管蠕动的中枢反射控制有关。本研究通过将跨突触逆行束路追踪与伪狂犬病病毒和烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH）组织化学相结合，确定了在控制食管蠕动的脑干回路中一氧化氮合酶的存在。
将病毒注射到15只大鼠中10只的食管内。60 - 63小时后，对脑切片进行病毒免疫荧光和NADPH组织化学处理。
荧光神经元标记仅限于疑核的致密部和孤束核的中央亚核。中央亚核中大多数荧光标记神经元对NADPH呈阳性染色（双重标记）。在致密部，几乎没有双重标记的神经元；然而，NADPH染色的终末围绕着荧光标记的运动神经元。
在孤束核中央亚核的运动前神经元中存在一氧化氮合酶，这些神经元支配疑核致密部的食管运动神经元。食管运动前神经元的胞体和终末中的NADPH染色表明，一氧化氮参与了中央亚核以及在疑核致密部食管运动前神经元与运动神经元之间突触接触部位的神经传递。
