睾丸精子胞浆内注射后的受精与妊娠情况
Fertilization and pregnancies following intracytoplasmic injection of testicular spermatozoa.
作者信息
Obruca A, Mock K, Feichtinger W, Lunglmayr G
机构信息
Institute for Sterility Treatment, Wien, Austria.
出版信息
J Assist Reprod Genet. 1995 Oct;12(9):627-31. doi: 10.1007/BF02212587.
PURPOSE
Intracytoplasmic injection (ICSI) with testicular sperm was performed in 16 couples. All men had ductal obstruction and failed previous attempts of epididymal sperm microaspiration.
METHODS
Testis tissue was obtained by excisional biopsies and incubated in HEPES buffered EBSS medium over 24 h at 37 degrees C. Motile sperm (Grade 1 to 2) were recovered in 13 patients and fertilized a total of 62 oozytes. Four pregnancies were achieved.
RESULTS
One healthy boy and two girls (twin pregnancy) were born.
CONCLUSIONS
The ongoing pregnancies revealed no fetal abnormalities on ultrasound scanning.
目的
对16对夫妇进行了睾丸精子胞浆内注射（ICSI）。所有男性均存在输精管阻塞，且先前的附睾精子微量抽吸尝试均失败。
方法
通过切除活检获取睾丸组织，并在37℃下于HEPES缓冲的EBSS培养基中孵育24小时以上。13例患者中回收了活动精子（1至2级），共使62个卵母细胞受精。获得了4次妊娠。
结果
一名健康男婴和两名女婴（双胎妊娠）出生。
结论
正在进行的妊娠在超声扫描中未发现胎儿异常。
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