Küpker W, al-Hasani S, Schulze W, Kühnel W, Schill T, Felberbaum R, Diedrich K
Department of Obstetrics and Gynecology, Medical University Lübeck, Germany.
J Assist Reprod Genet. 1995 Oct;12(9):620-6. doi: 10.1007/BF02212586.
Direct intracytoplasmic sperm injection (ICSI) is a successful treatment of severe male subfertility. Conventional in vitro fertilization shows poor pregnancy rates especially in cases of severe teratozoospermia. The objective was to determine if severe morphological defects of spermatozoa in oligoasthenoteratozoospermia (OAT) have any impact on the fertilization process in ICSI and if there are any specific morphological characteristics in nonfertilized oocytes after the ICSI procedure.
Ejaculates and nonfertilized oocytes were investigated by electron microscopy.
The lack of intracytoplasmic sperm-oocyte interaction, not severe sperm defects, displays the most critical role in the fertilization process. Clinical data with fertilization rates of 66% and pregnancy rates of 23.3% confirm the fertilization capacity of severely
直接卵胞浆内单精子注射（ICSI）是治疗严重男性生育力低下的一种成功方法。传统体外受精的妊娠率较低，尤其是在严重畸形精子症的病例中。目的是确定少弱畸精子症（OAT）中精子严重形态缺陷是否对ICSI受精过程有任何影响，以及ICSI术后未受精的卵母细胞是否有任何特定形态特征。
通过电子显微镜对射出的精液和未受精的卵母细胞进行研究。
在受精过程中，缺乏卵胞浆内精子-卵母细胞相互作用而非严重的精子缺陷起着最关键的作用。受精率为66%、妊娠率为23.3%的临床数据证实了严重……的受精能力
