Béarzotti M, Monnier A F, Vende P, Grosclaude J, de Kinkelin P, Benmansour A
INRA, unité de virologie et immunologie moléculaires, Jouy-en-Josas, France.
Vet Res. 1995;26(5-6):413-22.
In order to study the antigenic structure of the G protein of VHSV, we produced several anti-G monoclonal antibodies (MAbs) and used 4 neutralizing MAbs (NMAbs) to select resistant (MAR) mutants. Each MAR mutant was confronted with the 4 NMAbs in a neutralization test, and also with our panel of MAbs in surface plasmon resonance (SPR) analysis to determine the extent of their relatedness. Determination of the sequence of the entire G gene of representative MAR mutants allowed us to map the mutations responsible for the resistant phenotypes. We identified several locations on the G protein sequence, which represent, most probably, critical positions within the binding sites of the neutralizing MAbs. In addition, the MAR mutants selected with a cross-reactive MAb exhibited a reduced pathogenicity for fish. This indicated that the regions bearing the point mutations selected with MAb C10 were probably involved in the determination of the virulent phenotype.
为了研究病毒性出血性败血症病毒（VHSV）G蛋白的抗原结构，我们制备了几种抗G单克隆抗体（MAb），并使用4种中和单克隆抗体（NMAb）筛选抗性（MAR）突变体。每个MAR突变体在中和试验中与4种NMAb进行对抗，同时在表面等离子体共振（SPR）分析中与我们的单克隆抗体组进行对抗，以确定它们的相关程度。对代表性MAR突变体的整个G基因进行测序，使我们能够定位导致抗性表型的突变。我们在G蛋白序列上确定了几个位置，这些位置很可能代表中和单克隆抗体结合位点内的关键位置。此外，用交叉反应性单克隆抗体筛选出的MAR突变体对鱼类的致病性降低。这表明用单克隆抗体C10筛选出的携带点突变的区域可能参与了毒力表型的确定。
