Husain J, Juurlink B H
Department of Anatomy and Cell Biology, Saskatchewan Stroke Research Centre, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Brain Res. 1995 Nov 6;698(1-2):86-94. doi: 10.1016/0006-8993(95)00832-b.
Oligodendrocyte precursors and astrocytes in 2-week-old rat primary glial cultures survived 24 h of anoxia, suggesting both cell types could survive using glycolysis for ATP synthesis; however, when the hypoxia developed gradually, the majority of oligodendrocyte precursor cells died within 24 h of the beginning of the experiment but astrocytes survived. Similarly when cultures were exposed to an atmosphere of 1% oxygen, but not 2% or greater, oligodendrocyte precursors died within 24 h. Much more lipid peroxidation was seen under conditions of hypoxia than under conditions of anoxia suggesting that oligodendrocyte precursors died under the former condition because of free radical-induced damage. Using 5-(and -6)-carboxy-2',7'-dichlorodihydrofluorescein (DCFH) as an intracellular probe of oxidative stress, we have demonstrated directly on living cells that oligodendrocyte precursors have a poorer ability to scavenge free radicals than astrocytes. Furthermore, when free radicals were induced to form in the cells either by cysteine auto-oxidation or menadione redox cycling, oligodendrocyte precursors were more readily damaged than astrocytes. We conclude that oligodendroglial precursor cells are exquisitively sensitive to reactive oxygen species.
在2周龄大鼠的原代神经胶质培养物中，少突胶质前体细胞和星形胶质细胞在缺氧24小时后仍能存活，这表明这两种细胞类型都可以通过糖酵解来合成ATP以维持生存；然而，当缺氧逐渐发展时，大多数少突胶质前体细胞在实验开始后的24小时内死亡，但星形胶质细胞存活了下来。同样，当培养物暴露于含1%氧气的环境中，而不是2%或更高浓度的氧气环境时，少突胶质前体细胞在24小时内死亡。与缺氧条件相比，在低氧条件下观察到更多的脂质过氧化现象，这表明少突胶质前体细胞在低氧条件下死亡是由于自由基诱导的损伤。使用5 -（和 -6）-羧基 -2'，7'-二氯二氢荧光素（DCFH）作为细胞内氧化应激探针，我们在活细胞上直接证明了少突胶质前体细胞清除自由基的能力比星形胶质细胞差。此外，当通过半胱氨酸自动氧化或甲萘醌氧化还原循环诱导细胞内自由基形成时，少突胶质前体细胞比星形胶质细胞更容易受到损伤。我们得出结论，少突胶质前体细胞对活性氧极其敏感。
