Sann H, McCarthy P W, Jancsó G, Pierau F K
Max-Planck-Institut für physiologische und klinische Forschung, W.G. Kerckhoff-Institut, Parkstrasse 1, D-61231 Bad Nauheim, Germany.
Cell Tissue Res. 1995 Oct;282(1):155-61. doi: 10.1007/BF00319142.
The mouse monoclonal antibody RT97, which recognises the 200-kDa neurofilament subunit in its phosphorylated form, selectively labels the somata of sensory A-fibres (large light cells) in the dorsal root ganglion of the rat. We have tested the hypothesis that this antibody also visualises large diameter sensory fibres and their end structures in peripheral tissue, in particular in the skin. RT97 immunoreactivity is found in endings that are known to be served by myelinated afferent fibres, including Meissner-like endings, Merkel discs, hair follicle receptors, Pacinian corpuscles and free nerve endings. RT97 immunoreactivity has not, however, been observed in endings of presumably unmyelinated sensory fibres (intraepidermal fibres immunoreactive for substance P and calcitonin gene-related peptide) or in sympathetic fibres innervating sweat glands and blood vessels. In addition, neither systemic (100-150 mg/kg as adults) nor perineural capsaicin pre-treatment affects RT97 immunoreactivity in the skin. The data indicate that RT97 is a useful marker in the study of the capsaicin-insensitive sensory innervation of the skin and possibly other peripheral organs.
小鼠单克隆抗体RT97可识别磷酸化形式的200-kDa神经丝亚基，它能选择性地标记大鼠背根神经节中感觉A纤维（大型亮细胞）的胞体。我们检验了这样一个假说：该抗体也能显示外周组织，尤其是皮肤中，大直径感觉纤维及其终末结构。在已知由有髓传入纤维支配的终末中发现了RT97免疫反应性，这些终末包括类梅克尔小体、默克尔盘、毛囊感受器、环层小体和游离神经末梢。然而，在推测为无髓感觉纤维的终末（对P物质和降钙素基因相关肽呈免疫反应性的表皮内纤维）或支配汗腺和血管的交感纤维中未观察到RT97免疫反应性。此外，全身（成年大鼠为100-150mg/kg）或神经周围给予辣椒素预处理均不影响皮肤中的RT97免疫反应性。这些数据表明，RT97是研究皮肤以及可能其他外周器官中对辣椒素不敏感的感觉神经支配的有用标志物。
