McCarthy P W, Petts P, Hamilton A
AECC, Bournemouth, UK.
J Anat. 1992 Feb;180 ( Pt 1)(Pt 1):15-24.
The innervation of rat intervertebral disc and adjacent ligamentous tissue has been investigated using 2 antibodies, RT97 and anti-calcitonin gene-related peptide. Immunoreactivity to the peptide was found in many fibres throughout the long ligaments around the intervertebral discs and in the periosteum, especially associated with vascular channels entering the vertebral bodies. Few of the immunoreactive fibres entered the annular lamellae of the disc tissue. Most of those which terminated did so as fine fibres which lay close to, or in, the interlamellar spaces of the outer annulus fibrosus. Calcitonin gene-related peptide-like immunoreactivity was also found in more complex endings in the longitudinal ligaments and rarely within the annulus fibrosus. RT97-immunoreactivity was also present in the complex endings and associated fibres. Conversely, RT97-immunoreactivity was apparent only in a few fine filamentous fibre endings. This suggested that the majority of fine filamentous, or free, nerve endings were of an unmyelinated sensory origin. Alternatively, those endings of a more complex nature, which were RT97-immunoreactive, were of a myelinated sensory origin. No immunoreactivity to either antibody was seen in the inner annular or nuclear tissue. It was therefore concluded that the sensory innervation of the rat intervertebral disc has both myelinated and unmyelinated components, the latter being more extensive. Both types of innervation appear to be restricted to the outermost rings of the annulus fibrosus.
利用两种抗体，即RT97和抗降钙素基因相关肽，对大鼠椎间盘及相邻韧带组织的神经支配进行了研究。在椎间盘周围长韧带及骨膜的许多纤维中发现了对该肽的免疫反应性，尤其与进入椎体的血管通道相关。很少有免疫反应性纤维进入椎间盘组织的纤维环板层。那些终止的纤维大多以细纤维的形式终止，这些细纤维靠近或位于外层纤维环的层间间隙内。在纵韧带更复杂的终末中也发现了降钙素基因相关肽样免疫反应性，而在纤维环内则很少见。RT97免疫反应性也存在于复杂终末及相关纤维中。相反，RT97免疫反应性仅在少数细丝状纤维终末中明显。这表明大多数细丝状或游离神经终末起源于无髓感觉神经。或者，那些性质更复杂、对RT97有免疫反应性的终末起源于有髓感觉神经。在内层纤维环或髓核组织中未观察到对任何一种抗体的免疫反应性。因此得出结论，大鼠椎间盘的感觉神经支配既有有髓成分，也有无髓成分，后者更为广泛。两种类型的神经支配似乎都局限于纤维环的最外层。
